Singh, R B and Rao, V P and Sengar, R S (2023) Study of salinity induced oxidative stress and antioxidant responses in callus cultures of sugarcane. Ecological Genetics and Genomics, 26. ISSN 2405-9854
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Soil salinity adversely affects sugarcane growth and development by inducing impaired plant-water relations and osmotic imbalance. To understand the physio-biochemical response of sugarcane plants to salt (NaCl) stress, antioxidant enzymatic activity was analyzed in callus cultures of tolerant (BO 91) and susceptible (Co 0239) cultivars under in vitro conditions. Shoot tip explants were excised from the tolerant and susceptible plants and exposed to media without (2.2 ECw; control) and with (4.0, 6.0, 8.0, 10.0, 12.0, and 14.0 ECw) levels of salt for 90, 120, and 150 days. Significant effect of different salt concentrations on catalase (CAT), peroxidase (POX), nitrate reductase (NR), and indole-acetic acid (IAA) oxidase activities were evident from experimental observations. Under salinity stress conditions overall increasing trends of CAT and POX activity was found to be higher in callus cultures derived from tolerant cultivar compared to susceptible cultivar, indicates that antioxidant enzymes enhance cell protection against oxidative stress. Gradual decrease in NR and IAA oxidase activity with a corresponding increase in salt stress was less pronounced in callus of salt tolerant sugarcane than that of susceptible cultivar. A significant difference of soluble protein content was evident in callus cultures derived from both cultivars under varying degrees of salinity. Results of the present study showed that salt tolerant cultivar BO 91 has a more efficient defense mechanism and mitigates oxidative stress by maintaining higher anti-oxidant enzyme activity than the susceptible cultivar Co 0239. Based on multiple observations, it can be concluded that various biochemical and physiological mechanisms operated at cellular level in response to environmental stress in sugarcane. A comprehensive study of antioxidant enzymatic activity under in vitro salt stress conditions may prove to be a key breakthrough that has made a significant contribution to the literature and may guide other studies in the future.
| Item Type: | Article |
|---|---|
| Divisions: | Others |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | sugarcane, salinity, oxidative stress, antioxidant responses, callus cultures |
| Subjects: | Others > Soil Others > Plant Growth Others > Water Resources |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 09 Nov 2023 10:03 |
| Last Modified: | 09 Nov 2023 10:03 |
| URI: | http://oar.icrisat.org/id/eprint/12284 |
| Official URL: | https://www.sciencedirect.com/science/article/abs/... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
| Links: |
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