Gujjarlapudi, M and Kotarya, B and Mohanraj, S S and Gupta, D and Prasad, E R and Kalle, A M and Jaba, J and Ponnusamy, D and Padmasree, K (2023) Development of a rapid process for purification of Bowman-Birk and Kunitz inhibitors from legume seeds, and evaluation of their biophysical, insecticidal, and antimicrobial properties. International Journal of Biological Macromolecules (TSI), 238. ISSN 0141-8130
Full text not available from this repository. (Request a copy)Abstract
Bowman-Birk inhibitor (BBI ~10 kDa) and Kunitz inhibitor (KI ~20 kDa) are serine protease/proteinase inhibitor(s) [PI(s)] ubiquitously found in several Leguminous plant species with insecticidal and therapeutic properties. Due to narrow molecular mass differences, the separation of these inhibitors from a single seed variety is tedious. The present study is aimed to develop a rapid protocol (<24 h) for purifying BBI and KI from legume seeds using mild trichloroacetic acid (TCA) extraction followed by trypsin-affinity chromatography. The mature seeds of Vigna radiata and Cajanus platycarpus are used as a model to purify BBI and KI using this protocol. The BBI and KI purified from the seeds of V. radiata are labeled as VrBBI & VrKI, and C. platycarpus are labeled as CpBBI & CpKI, respectively. These PIs are confirmed by immunodetection and MALDI-TOF studies and further characterized for their structural (CD & fluorescence spectroscopy) and functional properties (temperature & DTT stability). BBI(s) purified using the above process are effective in the management of castor semi-looper ‘Achaea janata’, while KI(s) are effective in the management of pod borer ‘Helicoverpa armigera’. Besides, both BBI(s) and KI(s) have significant potential in controlling the growth of methicillin-sensitive ‘Staphylococcus aureus’, a gram-positive pathogenic bacterium.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Asia |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Bowman-Birk inhibitor, Kunitz inhibitor, narrow molecular mass, legume seeds |
| Subjects: | Others > Entomology Others > Legume Crops |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 07 Nov 2023 08:43 |
| Last Modified: | 07 Nov 2023 08:43 |
| URI: | http://oar.icrisat.org/id/eprint/12272 |
| Official URL: | https://www.sciencedirect.com/science/article/abs/... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | We thank Prof. Prakash Babu and Prof. Sarada Devi Tetali, University of Hyderabad for their critical suggestions in executing the research work. We especially thank Late Prof. Aparna Dutta Gupta from Dept. Animal Biology and Prof. Sarada Devi Tetali from Dept. Plant Sciences for generously extending their lab facilities. We thank the proteomics facility of the University of Hyderabad, Sandor Life Sciences, and the Centre for Cellular and Molecular Biology (CCMB) Hyderabad for helping in |
| Links: |
Actions (login required)
 |
View Item |
Altmetric
Altmetric