Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)

Jagadish, S V K and Muthurajan, R and Rang, Z W and Malo, R and Heuer, S and Bennett, J and Craufurd, P Q (2011) Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22). Rice, 4 (1). pp. 1-11.

PDF - Published Version Restricted to ICRISAT users only Download (594kB)

Abstract

In future climates, rice crops will be frequently exposed to water deficit and heat stress at the most sensitive flowering stage, causing spikelet sterility and yield losses. Water deficit alone and in combination with heat stress significantly reduced peduncle elongation, trapping 32% and 55% of spikelets within the leaf sheath, respectively. Trapped spikelets had lower spikelet fertility (66% in control) than those exserted normally (>93%). Average weighted fertility of exserted spikelets was lowest with heat stress (35%) but higher with combined stress (44%), suggesting acquired thermo-tolerance when preceded by water-deficit stress. Proteins favoring pollen germination, i.e., pollen allergens and beta expansin, were highly upregulated with water deficit but were at normal levels under combined stress. The chaperonic heat shock transcripts and proteins were significantly up-regulated under combined stress compared with either heat or water deficit. The importance of spikelet proteins responsive to water deficit and heat stress to critical physiological processes during flowering is discussed.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Flowering; Heat; Oryza sativa; Protein; Rice; Spikelet fertility; Water deficit
Subjects:	Others > Agriculture-Farming, Production, Technology, Economics
Depositing User:	Users 6 not found.
Date Deposited:	09 Sep 2011 08:38
Last Modified:	19 Feb 2014 05:45
URI:	http://oar.icrisat.org/id/eprint/1219
Official URL:	http://dx.doi.org/10.1007/s12284-011-9059-x
Projects:	UNSPECIFIED
Funders:	Felix Scholarship
Acknowledgement:	We thank the Felix Scholarship for funding the PhD of SVK Jagadish. The University of Reading and the International Rice Research Institute are thanked for providing facilities to support this research. USAID-BMGF (CSISA) are thanked for supporting the ongoing work on validation of these candidate genes. This research has been facilitated by access to the Australian Proteome Analysis Facility established under the Australian Government"s Major National Research Facilities Program. Bill Hardy from IRRI is thanked for editing the manuscript. The experiments comply with the current laws of Philippines.
Links:	Google Scholar Author

View Statistics

Actions (login required)

View Item