Immunity elicitors for induced resistance against the downy mildew pathogen in pearl millet

Lavanya, S N and Niranjan‑Raj, S and Jadimurthy, R and Sudarsan, S and Srivastava, R K and Tarasatyavati, C and Rajashekara, H and Gupta, V K (2022) Immunity elicitors for induced resistance against the downy mildew pathogen in pearl millet. Scientific Reports (TSI), 12. 01-17. ISSN 2045-2322

PDF Download (8MB)

Abstract

Pearl millet (Pennisetum glaucum (L.) R. Br.) is a globally important cereal whose production is severely constrained by downy mildew caused by Sclerospora graminicola (Sacc.). In this study, immunity eliciting properties of 3,5‑dichloroanthranilic acid (DCA), Cell Wall Glucan (CWG), Lipopolysaccharide (LPS), and Glycinebetaine (GB) was deciphered through enzymatic and protein studies based on elicitor treatment activated defense mechanisms. Glycinebetaine, LPS, CWS and DCA elicited enzyme activities and gene expression of the defense enzymes, such as β‑1,3‑glucanase, phenylalanine ammonia lyase (PAL), peroxidase (POX), polyphenol oxidase (PPO), lipoxygenase (LOX) and defense protein hydroxyproline‑rich glycoproteins (HRGPs). However, the speed and the extent of elicitation differed. High levels of enzyme activities and gene expression in elicitor‑treated P. glaucum positively correlated with the increased downy mildew resistance. A very rapid and large changes in elicitor-treated seedlings, in contrast to the delayed, smaller changes in the untreated susceptible control seedlings suggests that the rate and magnitude of defense gene expression are important for effective manifestation of defense against pathogen. As compared to other elicitors and control, GB promoted increase in enzyme activities and gene expression, implicating that GB is a promising elicitor of downy mildew resistance in P. glaucum.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	UNSPECIFIED
Uncontrolled Keywords:	downy mildew, pearl millet, Sclerospora graminicola, induced resistance, systemic acquired resistance, Induced systemic resistance
Subjects:	Mandate crops > Millets > Pearl Millet Others > Plant Pathology
Depositing User:	Mr Nagaraju T
Date Deposited:	20 Sep 2023 09:09
Last Modified:	20 Sep 2023 09:09
URI:	http://oar.icrisat.org/id/eprint/12186
Official URL:	https://www.nature.com/articles/s41598-022-07839-4
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors are grateful to ICAR (Indian Council of Agricultural Research), New Delhi, India for providing the financial support under AICRP scheme. The authors also thank the Institute of Excellence (IOE), Department of Science & Technology Promotion of University Research and Scientific Excellence (DST-PURSE), and Vijnana Bhavan, University of Mysore, for providing lab and instrumentation facilties. Mysore.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item