Transcriptome profiling reveals the expression and regulation of genes associated with Fusarium wilt resistance in chickpea (Cicer arietinum L.)

Garg, V and Chitikineni, A and Sharma, M and Ghosh, R and Samineni, S and Varshney, R K and Kudapa, H (2023) Transcriptome profiling reveals the expression and regulation of genes associated with Fusarium wilt resistance in chickpea (Cicer arietinum L.). The Plant Genome (TSI). 01-16. ISSN 1940-3372

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Abstract

Fusarium wilt (FW) is one of the most significant biotic stresses limiting chickpea production worldwide. To dissect the molecular mechanism of FW resistance in chickpea, comparative transcriptome analyses of contrasting resistance sources of chickpea genotypes under control and Fusarium oxysporum f. sp. ciceris (Foc) inoculated conditions were performed. The high-throughput transcriptome sequencing generated about 1137 million sequencing reads from 24 samples representing two resistant genotypes, two susceptible genotypes, and two near-isogenic lines under control and stress conditions at two-time points (7th- and 12th-day post-inoculation). The analysis identified 5182 differentially expressed genes (DEGs) between different combinations of chickpea genotypes. Functional annotation of these genes indicated their involvement in various biological processes such as defense response, cell wall biogenesis, secondary metabolism, and disease resistance. A significant number (382) of transcription factor encoding genes exhibited differential expression patterns under stress. Further, a considerable number of the identified DEGs (287) co-localized with previously reported quantitative trait locus for FWresistance. Several resistance/susceptibility-related genes, such as SERINE/THREONINE PROTEIN KINASE, DIRIGENT, and MLO exhibiting contrasting expression patterns in resistant and susceptible genotypes upon Foc inoculation, were identified. The results presented in the study provide valuable insights into the transcriptional dynamics associated with FW stress response in chickpea and provide candidate genes for the development of disease-resistant chickpea cultivars.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement Research Program : Asia
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Chickpea, Fusarium wilt resistance, Transcriptome profiling
Subjects:	Mandate crops > Chickpea
Depositing User:	Mr Nagaraju T
Date Deposited:	09 Jun 2023 10:08
Last Modified:	09 Jun 2023 10:08
URI:	http://oar.icrisat.org/id/eprint/12108
Official URL:	https://acsess.onlinelibrary.wiley.com/doi/epdf/10...
Projects:	UNSPECIFIED
Funders:	Science and Engineering Research Board
Acknowledgement:	The authors are thankful to Food Futures Institute, Murdoch University (Australia), for financial support. HB is thankful to the SERB of DST, Government of India, for providing the SERB Women Excellence Award (SB/WEA- 01/2017). This work was supported by resources provided by the Pawsey Supercomputing Research Centre with funding from the Australian Government and the Government of Western Australia.
Links:	Google Scholar

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