Identification of genomic regions linked to blast (Pyricularia grisea) resistance in pearl millet

Pujar, M and Kumar, S and Sharma, R and Ramu, P and Babu, R and Gupta, S K (2023) Identification of genomic regions linked to blast (Pyricularia grisea) resistance in pearl millet. Plant Breeding, 142 (4). pp. 506-517. ISSN 1439-0523

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Abstract

Blast disease causes serious economic yield losses in pearl millet. Identification and introgression of genomic regions associated with blast resistance can help to develop resistant cultivars to minimize yield losses incurred from blast outbreaks. In this study, 384 advanced pearl millet genotypes were screened against six blast pathotype-isolates (major pearl millet growing agro-ecologies of India), namely, Pg 45, Pg 118, Pg 138, Pg 186, Pg 204 and Pg 232. Analysis of variance showed significant (P < .001) variation among genotypes for blast reaction (susceptible to resistance). ICMR 08111 and ICMR 10888 genotypes showed resistance to all six blast pathotypes. A genome-wide association study performed with 264,241 single nucleotide polymorphic markers could successfully identify 15 SNPs (P = 1.26 x 10-7 to 9.22 x 10-12) underlying the genomic regions governing blast-resistance across five different chromosomes. The SNPs reported had a significant association in at least two of the three models tested (GLM, MLM and Farm CPU). These SNPs can be used in pearl millet-resistant breeding programmes after their function has been validated across different genetic backgrounds.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	UNSPECIFIED
Uncontrolled Keywords:	blast, general linear model (GLM), genome-wide association study (GWAS), linkage groups (LG), quantitative trait loci (QTL), single nucleotide polymorphisms (SNPs)
Subjects:	Mandate crops > Millets > Pearl Millet
Depositing User:	Mr Nagaraju T
Date Deposited:	05 Jun 2023 06:19
Last Modified:	03 Nov 2023 06:49
URI:	http://oar.icrisat.org/id/eprint/12103
Official URL:	https://onlinelibrary.wiley.com/doi/full/10.1111/p...
Projects:	Pearl Millet Hybrid Parent Research Consortium (PMHPRC)
Funders:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Acknowledgement:	Authors acknowledge the support provided by the Pearl Millet Hybrid Parent Research Consortium (PMHPRC), ICRISAT Patancheru - 502324 and CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC).
Links:	Google Scholar

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