Expression Dynamics of Genes and microRNAs at Different Growth Stages and Heat Treatments in Contrasting High Temperature Responsive Rice Genotypes

Bhogireddy, S and Babu, M S and Swamy, K N and Vishnukiran, T and Subrahmanyam, D and Sarla, N and Voleti, S R and Rao, P R and Mangrauthia, S K (2021) Expression Dynamics of Genes and microRNAs at Different Growth Stages and Heat Treatments in Contrasting High Temperature Responsive Rice Genotypes. Journal of Plant Growth Regulation, 41. pp. 74-91. ISSN 1435-8107

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Abstract

The global warming-driven climate change is becoming a major challenge for rice cultivation in Asia and Africa. High-temperature stress impairs the physiology and growth of rice plant, and ultimately results in reduced grain yield. This study was aimed to decipher the physiological and molecular changes occurring during different growth stages of heat-tolerant (N22) and -susceptible (Vandana) rice cultivars under three different heat treatments. Chlorophyll content, membrane integrity, gas exchange parameters and expression of genes and miRNAs were analyzed in N22 and Vandana at seedling, vegetative, and reproductive growth stages after exposing to short and long duration of high temperature stress, and recovery. A number of genes and miRNAs showed dynamic changes in their expression patterns at different growth stages and heat treatments, highlighting the necessity to understand gene regulation before employing the genes for modification through transgenic or gene editing approaches. Predominantly N22 showed distinct and unique capability to reprogram its physiological and molecular machinery during prolonged heat stress at reproductive stage, suggesting that the dynamics in gene regulation is crucial to determine its heat tolerant ability. The study has larger implications in deploying genes for the development of heat tolerant rice cultivars through breeding, transgenic, and genome editing approaches.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Heat stress, Oryza sativa, N22, Vandana, MDA, Photosynthesis
Subjects:	Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	17 Apr 2023 10:58
Last Modified:	17 Apr 2023 10:58
URI:	http://oar.icrisat.org/id/eprint/12068
Official URL:	https://link.springer.com/article/10.1007/s00344-0...
Projects:	Financial support received from Indian Council of Agricultural Research- NICRA (National Innovations in Climate Resilient Agriculture, 03 NICRA-030031 Grant in Aid) project is acknowledged.
Funders:	Indian Council of Agricultural Research- NICRA (National Innovations in Climate Resilient Agriculture)
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

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