Oral toxicity evaluation of probiotic strains isolated from Finger millet [Eleusine coracana (L.) Gaertn.] in Wistar rat models (in vivo)

Divisekera, D M and Samarasekera, J K R and Hettiarachchi, C and Maharjan, R and Gooneratne, J and Iqbal Choudhary, M and Gopalakrishnan, S and Wahab, A and Mazumdar, S D (2021) Oral toxicity evaluation of probiotic strains isolated from Finger millet [Eleusine coracana (L.) Gaertn.] in Wistar rat models (in vivo). Archives of Ecotoxicology, 3 (3). pp. 91-102. ISSN 2644-4747

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Abstract

This study evaluates the oral toxicity of five probiotic strains recently isolated from fermented flour of finger-millet (Eleusine coracana) varieties of Sri Lanka. Probiotic strains; Lactobacillus plantarum MF405176, Lactobacillus fermentum MF033346, Lactococcus lactis subspecies lactis MF480428, Enterococcus faecium MF480431and Pediococcus acidilactici MF480434 were evaluated for acute and sub-chronic oral toxicity in Wistars. Three individual doses (108 CFU/g, 1010 CFU/g and 1012 CFU/g) of each probiotic strain at single oral dose of 5000 mg/kg bw were orally administered to rats and observations were done till 14th day. Since no animals demonstrated signs of toxicity as a result of the administrated probiotics strains, repeated dose sub-chronic oral toxicity study was conducted by oral administration of three doses (108 CFU/g, 1010 CFU/g, 1012 CFU/g) of each probiotic strain at 1000 mg/kg bw/day for consecutive 90 days. Administration of probiotic strains to rats did not caused mortality in any of the tested doses. No changes in animal behavior, feed or water intake and negative effects on body weight observed. Probiotic feeding did not cause changes in analyzed biochemical and hematological parameters attributed to toxicity. Bacteremia, bacterial translocation and histopathological changes in rat organs were not observed. No significant difference in liver enzymes observed in treatment groups compared to control. In conclusion, all tested probiotic strains are nonpathogenic therefore could be considered as safe for human consumption.

Item Type:	Article
Divisions:	Global Research Program - Enabling Systems Transformation
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Finger-millet, Oral toxicity, Probiotics, Wistar rats
Subjects:	Mandate crops > Millets > Finger Millet Others > Food Processing & Technology
Depositing User:	Mr Arun S
Date Deposited:	17 Nov 2021 06:19
Last Modified:	17 Nov 2021 06:19
URI:	http://oar.icrisat.org/id/eprint/11924
Official URL:	https://doi.org/10.36547/ae.2021.3.3.91-102
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Financial assistance by the Indo-Sri Lanka Inter-Governmental Science and Technology Cooperation Programme Grant Number FP110. Centre for Science and Technology of the Non-Aligned and Other Developing Countries for awarding Joint NAM-ICCBS Post-graduate Research Fellowship for Divisekera Mudiyanselage Wasundara Devanmini Divisekera. Dr. Syed Mehmood Hasan, Professor of Pathology, Sindh Medical College, Jinnah Sindh Medical University, Pakistan for providing technical assistance in histopathological examinations.
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