Genome-wide transcriptome analysis and physiological variation modulates gene regulatory networks acclimating salinity tolerance in chickpea

Kumar, N and Soren, K R and Bharadwaj, C and Sneha Priya, P R and Shrivastava, A K and Pal, M and Roorkiwal, M and Kumar, K and Patil, B S and Soni, A and Nimmy, M S and Siddique, K H M and Varshney, R K (2021) Genome-wide transcriptome analysis and physiological variation modulates gene regulatory networks acclimating salinity tolerance in chickpea. Environmental and Experimental Botany (TSI), 187. pp. 1-14. ISSN 0098-8472

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Abstract

Salinity is a major abiotic stress that is a global threat to crop production, including chickpea. This study focused on understanding the complex molecular mechanisms underlying salinity tolerance using comparative transcriptome analysis of tolerant (ICCV 10, JG 11) and sensitive (DCP 92-3, Pusa 256) chickpea genotypes in control and salt-stressed environments. A total of 530 million reads were generated from root samples of four genotypes using Illumina HiSeq-2500. A total of 21,698 differentially expressed genes (DEGs) were identified, of which 11,456 and 10,242 were up- and down-regulated, respectively, in comparative analysis. These DEGs were associated with crucial metabolic pathways, including hormone signaling, photosynthesis, lipid and carbohydrate metabolism, and cell wall biogenesis. Gene ontology (GO) examination revealed an enrichment of transcripts involved in salinity response. A total of 4257 differentially expressed GO terms were categorized into 64 functional groups; of which, GO terms like, integral component of membrane, organelle, and cellular anatomical entity were highly represented in tolerant genotypes under salt stress. Significant up-regulation of transcripts encoding potassium transporter family HAK/KUP proteins, MIP/aquaporin protein family, NADH dehydrogenase, pectinesterase, and PP2C family proteins occurred under salt stress. The tolerant lines (ICCV 10 and JG 11) engaged highly efficient machinery in response to elevated salt stress, especially for signal transduction, transport and influx of K+ ions, and osmotic homeostasis. The overall study highlights the role of potential candidate genes and their regulatory networks which can be utilized in breeding salt tolerant chickpea cultivars.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Chickpea, Differential gene expression, Salinity, Transcriptomics
Subjects:	Mandate crops > Chickpea Others > Genetics and Genomics
Depositing User:	Mr Arun S
Date Deposited:	29 Aug 2021 09:55
Last Modified:	29 Aug 2021 09:55
URI:	http://oar.icrisat.org/id/eprint/11887
Official URL:	https://doi.org/10.1016/j.envexpbot.2021.104478
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors acknowledge to the Farm Unit Indian Council of Agricultural Research (ICAR) – Indian Agricultural Research Institute (IARI) and ICAR – National Phytotron Facility for, providing the facilities to carry out the experiments and funding agencies ICAR-IARI Fellowship, Department of Biotechnology (DBT)-AISRF and ICAR-IRA for partially providing funds
Links:	Google Scholar

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