Molecular Genetic Diversity and Population Structure in Ethiopian Chickpea Germplasm Accessions

Getahun, T and Tesfaye, K and Fikre, A and Haileslassie, T and Chitikineni, A and Thudi, M and Varshney, R K (2021) Molecular Genetic Diversity and Population Structure in Ethiopian Chickpea Germplasm Accessions. Diversity (TSI), 13 (6). pp. 1-15. ISSN 1424-2818

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Abstract

Chickpea (Cicer arietinum L.) is a cheap source of protein and rich in minerals for people living in developing countries. In order to assess the existing molecular genetic diversity and determine population structures in selected Ethiopian chickpea germplasm accessions (118), a set of 46 simple sequence repeat (SSR) markers equally distributed on the chickpea genome were genotyped. A total of 572 alleles were detected from 46 SSR markers, and the number of alleles per locus varied from 2 (ICCM0289) to 28 (TA22). The average number of alleles per locus, polymorphism information content, and expected heterozygosity were 12, 0.684, and 0.699, respectively. Phylogenetic analysis grouped the 118 chickpea genotypes from diverse sources into three evolutionary and/or biological groups (improved desi, improved kabuli, and landraces). The population structure analysis revealed six sub-populations from 118 chickpea genotypes studied. AMOVA revealed that 57%, 29%, and 14% of the total genetic variations were observed among individuals, within populations, and among populations. The insights into the genetic diversity at molecular levels in the Ethiopian germplasm lines can be used for designing conservation strategies as well as the diverse germplasm lines identified in this study can be used for trait dissection and trait improvement.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	Chickpea, Microsatellite markers, Genetic diversity, Population structure
Subjects:	Mandate crops > Chickpea Others > Genetics and Genomics
Depositing User:	Mr Arun S
Date Deposited:	23 Aug 2021 07:39
Last Modified:	23 Aug 2021 07:39
URI:	http://oar.icrisat.org/id/eprint/11877
Official URL:	https://doi.org/10.3390/d13060247
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	T.G. is sincerely thankful to the Institute of Biotechnology, Addis Ababa University, for financial support. R.K.V. greatly acknowledges the funding support from the Bill and Melinda Gates Foundation, Tropical Legumes III. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR Consortium.
Links:	Google Scholar

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