A diagnostic marker kit for Fusarium wilt and sterility mosaic diseases resistance in pigeonpea

Saxena, R K and Hake, A and Bohra, A and Khan, A W and Hingane, A and Sultana, R and Singh, I P and Naik, S J S and Varshney, R K (2020) A diagnostic marker kit for Fusarium wilt and sterility mosaic diseases resistance in pigeonpea. Theoretical and Applied Genetics (TSI). ISSN 0040-5752

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Abstract

Fusarium wilt (FW) and sterility mosaic diseases (SMD) are key biotic constraints to pigeonpea production. Occurrence of these two diseases in congenial conditions is reported to cause complete yield loss in susceptible pigeonpea cultivars. Various studies to elucidate genomic architecture of the two traits have revealed significant marker–trait associations for use in breeding programs. However, these DNA markers could not be used effectively in genomics-assisted breeding for developing FW and SMD resistant varieties primarily due to pathogen variability, location or background specificity, lesser phenotypic variance explained by the reported QTL and cost-inefficiency of the genotyping assays. Therefore, in the present study, a novel approach has been used to develop a diagnostic kit for identification of suitable FW and SMD resistant lines. This kit was developed with 10 markers each for FW and SMD resistance. Investigation of the diversity of these loci has shown the role of different alleles in different resistant genotypes. Two genes (C.cajan_03691 and C.cajan_18888) for FW resistance and four genes (C.cajan_07858, C.cajan_20995, C.cajan_21801 and C.cajan_17341) for SMD resistance have been identified. More importantly, we developed a customized and cost-effective Kompetitive allele-specific PCR genotyping assay for the identified genes in order to encourage their downstream applications in pigeonpea breeding programs. The diagnostic marker kit developed here will offer great strength to pigeonpea varietal development program, since the resistance against these two diseases is essentially required for nominating an improved line in varietal release pipeline.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	Pigeonpea, Plant Disease, Genomics
Subjects:	Mandate crops > Pigeonpea Others > Genetics and Genomics Others > Plant Disease
Depositing User:	Mr Arun S
Date Deposited:	15 Nov 2020 07:56
Last Modified:	15 Nov 2020 07:56
URI:	http://oar.icrisat.org/id/eprint/11657
Official URL:	https://doi.org/10.1007/s00122-020-03702-0
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Authors are thankful to the Department of Agriculture Cooperation & Farmers Welfare, Ministry of Agriculture & Farmers Welfare, Government of India and Bill and Melinda Gates Foundation for financial support. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR Consortium.
Links:	Google Scholar

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