Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence

Kiranmayee, K N S U and Hash, C T and Sivasubramani, S and Ramu, P and Amindala, B P and Rathore, A and Kishor, P B K and Gupta, R and Deshpande, S P (2020) Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence. Genes (TSI), 11 (1026). pp. 1-26. ISSN 2073-4425

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Abstract

This study was conducted to dissect the genetic basis and to explore the candidate genes underlying one of the important genomic regions on an SBI-10 long arm (L), governing the complex stay-green trait contributing to post-flowering drought-tolerance in sorghum. A fine-mapping population was developed from an introgression line cross—RSG04008-6 (stay-green) � J2614-11 (moderately senescent). The fine-mapping population with 1894 F2 was genotyped with eight SSRs and a set of 152 recombinants was identified, advanced to the F4 generation, field evaluated with three replications over 2 seasons, and genotyped with the GBS approach. A high-resolution linkage map was developed for SBI-10L using 260 genotyping by sequencing—Single Nucleotide Polymorphism (GBS–SNPs). Using the best linear unpredicted means (BLUPs) of the percent green leaf area (%GL) traits and the GBS-based SNPs, weidentified seven quantitative trait loci (QTL) clusters and single gene, mostly involved in drought-tolerance, for each QTL cluster, viz., AP2/ERF transcription factor family (Sobic.010G202700), NBS-LRR protein (Sobic.010G205600), ankyrin-repeat protein (Sobic.010G205800), senescence-associated protein (Sobic.010G270300), WD40 (Sobic.010G205900), CPK1 adapter protein (Sobic.010G264400), LEA2 protein (Sobic.010G259200) and an expressed protein (Sobic.010G201100). The target genomic region was thus delimited from 15 Mb to 8 genes co-localized with QTL clusters, and validated using quantitative real-time (qRT)–PCR.

Item Type: Article
Divisions: Research Program : Genetic Gains
Statistics, Bio-Informatics & Data Management
CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords: sorghum chromosome 10 long arm (SBI-10L), fine-mapping, Stay-green, Candidate genes, Percent green leaf area, Delayed senescence
Subjects: Mandate crops > Sorghum
Others > Genetics and Genomics
Depositing User: Mr Arun S
Date Deposited: 13 Nov 2020 05:42
Last Modified: 13 Nov 2020 05:42
URI: http://oar.icrisat.org/id/eprint/11643
Official URL: https://doi.org/10.3390/genes11091026
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This work is a part of the Ph.D. and post-doctoral research work of the first author. The authors are thankful to C. Muralidhar for his help in conducting field experiments. Funding support to R.G. and S.P.D. from the Department of Biotechnology (grant# BT-IN-UK-VNC-42-RG-2015-16), Government of India, the Biotechnology and Biological Sciences Research Council (GCRF/BBSRC grant # BB/P027970/1), United Kingdom, and the CGIAR Research Program on Grain Legumes and Dryland Cereals (CRP-GLDC) is gratefully acknowledged. ICRISAT is a member of the CGIAR consortium. PBK is grateful to the VFSTR, Guntur, for providing fellowship.
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