Genotyping-by-sequencing and multilocation evaluation of two interspecific backcross populations identify QTLs for yield-related traits in pigeonpea

Saxena, R K and Kale, S and Mir, R R and Mallikarjuna, N and Yadav, P and Das, R R and Molla, J and Sonnappa, M and Ghanta, A and Narasimhan, Y and Rathore, A and Kumar, C V S and Varshney, R K (2019) Genotyping-by-sequencing and multilocation evaluation of two interspecific backcross populations identify QTLs for yield-related traits in pigeonpea. Theoretical and Applied Genetics (TSI), 133 (3). pp. 737-749. ISSN 0040-5752

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Abstract

This study has identified single-nucleotide polymorphism (SNP) markers associated with nine yield-related traits in pigeonpea by using two backcross populations (BP) developed through interspecific crosses and evaluating them at two locations and 3 years. In both the populations, markers have shown strong segregation distortion; therefore, a quantitative trait locus (QTL) mapping mixed model was used. A total of 86 QTLs explaining 12–21% phenotypic variation were detected in BP-1. On the other hand, 107 QTLs explaining 11–29% phenotypic variation were detected in BP-2. Although most QTLs were environment and trait specific, few stable and consistent QTLs were also detected. Interestingly, 11 QTLs in BP-2 were associated with more than one trait. Among these QTLs, eight QTLs associated with days to 50% flowering and days to 75% maturity were located on CcLG07. One SNP “S7_14185076” marker in BP-2 population has been found associated with four traits, namely days to 50% flowering, days to 75% maturity, primary branches per plant and secondary branches per plant with positive additive effect. Hence, the present study has not only identified QTLs for yield-related traits, but also discovered novel alleles from wild species, which can be used for improvement of traits through genomics-assisted breeding.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	Pigeonpea, Genetics, Genomics
Subjects:	Mandate crops > Pigeonpea Others > Genetics and Genomics
Depositing User:	Mr Arun S
Date Deposited:	08 Sep 2020 05:59
Last Modified:	08 Sep 2020 05:59
URI:	http://oar.icrisat.org/id/eprint/11613
Official URL:	https://doi.org/10.1007/s00122-019-03504-z
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Authors are thankful for the Department of Agriculture Cooperation & Farmers’ Welfare, Ministry of Agriculture & Farmers Welfare, Government of India and United States Agency for International Development (USAID). This work is also partially funded as part of the initiative “Adapting Agriculture to Climate Change: Collecting, Protecting and Preparing Crop Wild Relatives,” which is supported by the Government of Norway. The project is managed by the Global Crop Diversity Trust. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR Consortium.
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