Molecular and Physiological Alterations in Chickpea under Elevated CO2 Concentrations

    Palit, P and Ghosh, R and Tolani, P and Tarafdar, A and Chitikineni, A and Bajaj, P and Sharma, M and Kudapa, H and Varshney, R K (2020) Molecular and Physiological Alterations in Chickpea under Elevated CO2 Concentrations. Plant and Cell Physiology (TSI), 61 (8). pp. 1449-1463. ISSN 1471-9053

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    Abstract

    The present study reports profiling of the elevated carbon dioxide (CO2) concentration responsive global transcriptome in chickpea, along with a combinatorial approach for exploring interlinks between physiological and transcriptional changes, important for the climate change scenario. Various physiological parameters were recorded in two chickpea cultivars (JG 11 and KAK 2) grown in open top chambers under ambient [380 parts per million (ppm)] and two stressed/elevated CO2 concentrations (550 and 700 ppm), at different stages of plant growth. The elevated CO2 concentrations altered shoot and root length, nodulation (number of nodules), total chlorophyll content and nitrogen balance index, significantly. RNA-Seq from 12 tissues representing vegetative and reproductive growth stages of both cultivars under ambient and elevated CO2 concentrations identified 18,644 differentially expressed genes including 9,687 transcription factors (TF). The differential regulations in genes, gene networks and quantitative real-time polymerase chain reaction (qRT-PCR) -derived expression dynamics of stress-responsive TFs were observed in both cultivars studied. A total of 138 pathways, mainly involved in sugar/starch metabolism, chlorophyll and secondary metabolites biosynthesis, deciphered the crosstalk operating behind the responses of chickpea to elevated CO2 concentration.

    Item Type: Article
    Divisions: Research Program : Asia
    Research Program : Genetic Gains
    CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
    Uncontrolled Keywords: Climate change, Differentially expressed genes, Elevated CO2 concentration, RNA-Seq, Stress pathways, Transcriptome
    Subjects: Mandate crops > Chickpea
    Others > Genetics and Genomics
    Others > Climate Change
    Depositing User: Mr Arun S
    Date Deposited: 04 Sep 2020 12:47
    Last Modified: 04 Sep 2020 12:47
    URI: http://oar.icrisat.org/id/eprint/11585
    Official URL: https://doi.org/10.1093/pcp/pcaa077
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: Authors are thankful to the Department of Biotechnology, Department of Science and Technology (DST, Climate Change Program) of Government of India, and Bill & Melinda Gates Foundation for supporting this study partially. P.P. acknowledges Women Scientist Scheme of Department of Science and Technology, Government of India (SR/WOS-A/ LS-1175/2015). R.K.V. is thankful to the Science & Engineering Research Board (SERB) of DST, Government of India for providing the J C Bose National Fellowship (SB/S9/Z-13/2019) and European Commission for funding the 3-year-long ERASMUSþ—Capacity Building project entitled ‘Strengthening education, research and innovation for climate-smart crops in India’ (598797-EPP-1-2018-1-ELEPPKA2- CBHE-JP). This work has been undertaken as part of CGIAR Research Program on Grain Legumes & Dryland Cereals. ICRISAT is a member of CGIAR Research Consortium.
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