Seed protein content and its relationships with agronomic traits in pigeonpea is controlled by both main and epistatic effects QTLs

Obala, J and Saxena, R K and Singh, V K and Kale, S M and Garg, V and Kumar, C V S and Saxena, K B and Tongoona, P and Sibiya, J and Varshney, R K (2020) Seed protein content and its relationships with agronomic traits in pigeonpea is controlled by both main and epistatic effects QTLs. Scientific Reports (TSI), 10 (1). pp. 1-17. ISSN 2045-2322

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Abstract

The genetic architecture of seed protein content (SPC) and its relationships to agronomic traits in pigeonpea is poorly understood. Accordingly, five F2 populations segregating for SPC and four agronomic traits (seed weight (SW), seed yield (SY), growth habit (GH) and days to first flowering (DFF)) were phenotyped and genotyped using genotyping-by-sequencing approach. Five high-density population-specific genetic maps were constructed with an average inter-marker distance of 1.6 to 3.5 cM, and subsequently, integrated into a consensus map with average marker spacing of 1.6 cM. Based on analysis of phenotyping data and genotyping data, 192 main effect QTLs (M-QTLs) with phenotypic variation explained (PVE) of 0.7 to 91.3% were detected for the five traits across the five populations. Major effect (PVE ≥ 10%) M-QTLs included 14 M-QTLs for SPC, 16 M-QTLs for SW, 17 M-QTLs for SY, 19 M-QTLs for GH and 24 M-QTLs for DFF. Also, 573 epistatic QTLs (E-QTLs) were detected with PVE ranging from 6.3 to 99.4% across traits and populations. Colocalization of M-QTLs and E-QTLs explained the genetic basis of the significant (P < 0.05) correlations of SPC with SW, SY, DFF and GH. The nature of genetic architecture of SPC and its relationship with agronomic traits suggest that genomics-assisted breeding targeting genome-wide variations would be effective for the simultaneous improvement of SPC and other important traits.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	Pigeonpea, Genetics, Genomics, Nutrition
Subjects:	Mandate crops > Pigeonpea Others > Genetics and Genomics Others > Food and Nutrition
Depositing User:	Mr Arun S
Date Deposited:	04 Sep 2020 12:42
Last Modified:	04 Sep 2020 12:42
URI:	http://oar.icrisat.org/id/eprint/11583
Official URL:	https://doi.org/10.1038/s41598-019-56903-z
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Authors are thankful to the United States Agency for International Development (USAID) and Department of Agriculture Cooperation & Farmers Welfare, Ministry of Agriculture & Farmers Welfare, Government of India; for funding support. We are thankful to Vinay Kumar, Suryanayana Vechalapu and Meriga Sudhakar for the great technical assistance offered. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR Consortium.
Links:	Google Scholar

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