High resolution mapping of restoration of fertility (Rf) by combining large population and high density genetic map in pigeonpea [Cajanus cajan (L.) Millsp]

    Saxena, R K and Molla, J and Yadav, P and Varshney, R K (2020) High resolution mapping of restoration of fertility (Rf) by combining large population and high density genetic map in pigeonpea [Cajanus cajan (L.) Millsp]. BMC Genomics (TSI), 21 (1). pp. 1-8. ISSN 1471-2164

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    Abstract

    Background: Restoration of fertility (Rf) is an important trait for pigeonpea hybrid breeding. Few coarse quantitative trait locus (QTL) studies conducted in the past identified QTLs with large confidence intervals on the genetic map and could not provide any information on possible genes responsible for Rf in pigeonpea. Therefore, a larger population comprising of 369 F2s derived from ICPA 2039 × ICPL 87119 was genotyped with high density Axiom Cajanus SNP Array with 56 K single nucleotide polymorphism (SNPs) for high resolution mapping of Rf. Results: A genetic map with 4867 markers was developed and a total of four QTLs for Rf were identified. While one major effect QTL (qRf8.1) was co-localized with the QTL identified in two previous studies and its size was refined from 1.2 Mb to 0.41 Mb. Further analysis of qRf8.1 QTL with genome sequence provided 20 genes including two genes namely flowering locus protein T and 2-oxoglutarate/Fe (II)-dependent dioxygenases (2-ODDs) superfamily protein with known function in the restoration of fertility. Conclusion: The qRf8.1 QTL and the potential candidate genes present in this QTL will be valuable for genomicsassisted breeding and identification of causal genes/nucleotides for the restoration of fertility in the hybrid breeding program of pigeonpea.

    Item Type: Article
    Divisions: Research Program : Genetic Gains
    CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
    Uncontrolled Keywords: Pigeonpea, Hybrids, Restoration of fertility, Genetic map, QTL, Candidate gene
    Subjects: Mandate crops > Pigeonpea
    Others > Genetics and Genomics
    Depositing User: Mr Arun S
    Date Deposited: 09 Aug 2020 09:12
    Last Modified: 09 Aug 2020 09:12
    URI: http://oar.icrisat.org/id/eprint/11545
    Official URL: https://doi.org/10.1186/s12864-020-06859-6
    Projects: Tropical Legumes Project TLII
    Funders: Department of Agriculture Cooperation & Farmers Welfare, Ministry of Agriculture & Farmers Welfare, Government ofIndia, Department of Biotechnology, Government of India, Tropical Legumes Project TLII from Bill and Melinda Gates Foundation
    Acknowledgement: This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR Consortium. Authors would also like to thank Mr. Suryanarayana and Mr. Sudhakar for the technical help.
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