Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance

Yu, B and Jiang, H and Pandey, M K and Huang, L and Huai, D and Zhou, X and Kang, Y and Varshney, R K and Sudini, H and Ren, X and Luo, H and Liu, N and Chen, W and Guo, J and Li, W and Ding, Y and Jiang, Y and Lei, Y and Liao, B (2020) Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance. Toxins (TSI), 12 (156). pp. 1-16. ISSN 2072-6651

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Aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) are the most common aflatoxins produced by Aspergillus flavus in peanuts, with high carcinogenicity and teratogenicity. Identification of DNA markers associated with resistance to aflatoxin production is likely to o�er breeders e�cient tools to develop resistant cultivars through molecular breeding. In this study, seeds of 99 accessions of a Chinese peanut mini-mini core collection were investigated for their reaction to aflatoxin production by a laboratory kernel inoculation assay. Two resistant accessions (Zh.h0551 and Zh.h2150) were identified, with their aflatoxin content being 8.11%–18.90% of the susceptible control. The 99 peanut accessions were also genotyped by restriction site-associated DNA sequencing (RAD-Seq) for a genome-wide association study (GWAS). A total of 60 SNP (single nucleotide polymorphism) markers associated with aflatoxin production were detected, and they explained 16.87%–31.70% of phenotypic variation (PVE), with SNP02686 and SNP19994 possessing 31.70% and 28.91% PVE, respectively. Aflatoxin contents of accessions with “AG” (existed in Zh.h0551 and Zh.h2150) and “GG” genotypes of either SNP19994 or SNP02686 were significantly lower than that of “AA” genotypes in the mean value of a three-year assay. The resistant accessions and molecular markers identified in this study are likely to be helpful for deployment in aflatoxin resistance breeding in peanuts.

Item Type: Article
Divisions: Research Program : Asia
Research Program : Genetic Gains
Uncontrolled Keywords: Aflatoxin resistance, Peanut, Genome-wide association study (GWAS), Mini-mini core collection, Restriction site associated DNA sequencing (RAD-Seq)
Subjects: Mandate crops > Groundnut
Others > Oilseeds
Others > Genetics and Genomics
Others > Aflatoxins
Depositing User: Mr Arun S
Date Deposited: 18 May 2020 11:38
Last Modified: 18 May 2020 11:40
Official URL:
Funders: National Natural Science Foundation of China, National Key R&D Program of China, Crop Germplasm Resources Protection Project of Ministry of Agriculture, Rural Affairs of the People’s Republic of China, Plant Germplasm Resources Sharing Platform, China Agriculture Research System
Acknowledgement: UNSPECIFIED
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