Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

Wan, L and Lei, Y and Yan, L and Liu, Y and Pandey, M K and Wan, X and Varshney, R K and Fang, J and Liao, B (2020) Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant. BMC Plant Biology (TSI), 20 (1). pp. 2-19. ISSN 1471-2229

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Abstract

Background: Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut can enhance the processing efficiency and quality of peanut oil. An integrative analysis of transcriptomes, metabolomes and histocytology was performed on wsc mutant and its wild type to investigate the regulatory mechanisms underlying color pigmentation. Result: Metabolomes revealed flavonoids were redirected in wsc, while multi-omics analyses of wsc mutant seeds and testae uncovered WSC influenced the flavonoids biosynthesis in testa as well as suberin formation, glycolysis, the TCA cycle and amino acid metabolism. The mutation also enhanced plant hormones synthesis and signaling. Further, co-expression analysis showed that FLS genes co-expressed with MBW complex member genes. Combining tissue expression patterns, genetic analyses, and the annotation of common DEGs for these three stages revealed that three testa specific expressed candidate genes, Araip.M7RY3, Aradu.R8PMF and Araip.MHR6K were likely responsible for the white testa phenotype. WSC might be regulated expression competition between FLS and DFR by controlling hormone synthesis and signaling as well as the MBW complex. Conclusions: The results of this study therefore provide both candidate genes and novel approaches that can be applied to improve peanut with desirable seed coat color and flavonoid quality.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Groundnut, Legumes, Genetics, Genomics
Subjects:	Mandate crops > Groundnut Others > Oilseeds Others > Genetics and Genomics Others > Legume Crops
Depositing User:	Mr Arun S
Date Deposited:	15 May 2020 09:54
Last Modified:	15 May 2020 09:54
URI:	http://oar.icrisat.org/id/eprint/11508
Official URL:	https://doi.org/10.1186/s12870-020-02383-7
Projects:	UNSPECIFIED
Funders:	National Natural Science Foundation of China, The Innovation Program of the Chinese Academy of Agricultural Sciences, The Central Public-Interest Scientific Institution Basal Research Fund, China Agriculture Research System, The National Key Research and Development Program of China, The Jiangxi Agriculture Research System
Acknowledgement:	We thank Dr. Juncheng Zhang (Huazhong Agricultural University, China) for critically revising the English in this paper, as well as Dr. Jianbo Jian (BGI) for assistance with RNA-seq analyses.
Links:	Google Scholar

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