Identification of QTLs and candidate genes for high grain Fe and Zn concentration in sorghum [Sorghum bicolor (L.)Moench]

Kotla, A and Phuke, R and Hariprasanna, K and Mehtre, S P and Rathore, A and Gorthy, S and Srivastava, R K and Das, R and Bhanu Prakash, A and Radhika, K and Hash, C T and Reddy, B V S and Patil, J V and Jabeen, F and Shashikanth, D and Jaganathan, J and Gaddameedi, A and Subhasini, V and Deshpande, S and Kumar, A A (2019) Identification of QTLs and candidate genes for high grain Fe and Zn concentration in sorghum [Sorghum bicolor (L.)Moench]. Journal of Cereal Science (TSI), 90. pp. 1-9. ISSN 0733-5210

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Abstract

Sorghum is a major food crop in the semi-arid tropics of Africa and Asia. Enhancing the grain iron (Fe) and zinc (Zn) concentration in sorghum using genetic approaches would help alleviate micronutrient malnutrition in millions of poor people consuming sorghum as a staple food. To localize genomic regions associated with grain Fe and Zn, a sorghum F6 recombinant inbred line (RIL) population (342 lines derived from cross 296B PVK 801) was phenotyped in six environments, and genotyped with simple sequence repeat (SSR), DArT (Diversity Array Technology) and DArTSeq (Diversity Array Technology) markers. Highly significant genotype environment interactions were observed for both micronutrients. Grain Fe showed greater variation than Zn. A sorghum genetic map was constructed with 2088 markers (1148 DArTs, 927 DArTSeqs and 13 SSRs) covering 1355.52 cM with an average marker interval of 0.6 cM. Eleven QTLs (individual) and 3 QTLs (across) environments for Fe and Zn were identified. We identified putative candidate genes from the QTL interval of qfe7.1, qzn7.1, and qzn7.2 (across environments) located on SBI-07 involved in Fe and Zn metabolism. These were CYP71B34, and ZFP 8 (ZINC FINGER PROTEIN 8). After validation, the linked markers identified in this study can help in developing high grain Fe and Zn sorghum cultivars in sorghum improvement programs globally.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Biofortification, RIL, QTL, Iron and zinc, Candidate genes, Sorghum
Subjects:	Mandate crops > Sorghum Others > Genetics and Genomics Others > Food and Nutrition
Depositing User:	Mr Arun S
Date Deposited:	08 Apr 2020 12:32
Last Modified:	08 Apr 2020 12:32
URI:	http://oar.icrisat.org/id/eprint/11467
Official URL:	https://doi.org/10.1016/j.jcs.2019.102850
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors thank the funding support by Department of Biotechnology, Government of India for financing the Project Biofortifying sorghum with high grain iron and zinc concentration for combating micronutrient malnutrition” vide No BT/PR 1630/AGR/2/806/2011 Dt: 28 February 2012. We would also like to thank the Charles Renard Analytical Laboratory for analyzing grain iron and zinc concentration of samples. Thanks are also due to Senthivel, Manish Vishwakarma, Manish Pandey and Rajeev Varshney for their guidance in interpreting results. We dedicate this research work to Dr Belum Venkata Subba Reddy, who left for heavenly abode after devoting much of his life for sorghum improvement research.
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