Velu, G and Kulkarni, V N and Muralidharan, V and Rai, K N and Longvah, T and Sahrawat, K L and Raveendran, T S (2006) A rapid screening method for grain iron content in Pearl Millet. International Sorghum and Millets Newsletter, 47. pp. 158-161.
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Abstract
Enhancing grain iron (Fe) content is one of the effective ways of increasing the Fe intake and reducing the incidence of Fe-deficiency anaemia (Welch and Graham 2002). Large genetic variability for grain Fe content has been reported in many crops (Graham et al. 1999). Pearl millet [Pennisetum glaucum (L.) R.Br.] is a major source of dietary energy for millions of people living in the arid and semi-arid tropical regions of Africa and Asia. It has on an average 50 mg kg-1 of grain Fe, which is more than wheat, rice and maize. Studies with limited germplasm have shown large genetic variability for this trait, indicating good opportunities to select and/or breed millet genotypes with still higher grain Fe (Jambunathan and Subramanian 1988; Abdalla et al. 1998). The bottleneck in this process is the high cost of Fe estimation. At present Fe estimation is done with digests using Atomic Absorption Spectrophotometry (AAS) and Inductively Coupled Plasma-Atomic Absorption Spectrophotometry (ICP), which require relatively expensive instruments and are time-consuming. These chemical analyses would be prohibitively costly for evaluating a large number of progenies during the course of a breeding program. A procedure based on Perls’ Prussian blue stain was proposed for rapid screening of grain Fe content in rice (Prom-u-thai et al. 2003; Krishnan et al. 2003) which involves scoring color intensity in the embryo of cut and treated seeds (with 2% Prussian blue) through a stereomicroscope. The objective of this research was to simplify the method and assess its effectiveness in screening for grain Fe content in pearl millet.
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Mandate crops > Millets |
| Depositing User: | Library ICRISAT |
| Date Deposited: | 11 Sep 2011 10:56 |
| Last Modified: | 11 Sep 2011 10:56 |
| URI: | http://oar.icrisat.org/id/eprint/1143 |
| Official URL: | |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
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