Exploiting plant growth-promoting Amycolatopsis sp. for bio-control of charcoal rot of sorghum (Sorghum bicolor L.) caused by Macrophomina phaseolina (Tassi) Goid

    Gopalakrishnan, S and Srinivas, V and Naresh, N and Alekhya, G and Sharma, R (2019) Exploiting plant growth-promoting Amycolatopsis sp. for bio-control of charcoal rot of sorghum (Sorghum bicolor L.) caused by Macrophomina phaseolina (Tassi) Goid. Archives of Phytopathology and Plant Protection(TSI), 52 (7-8). pp. 543-559. ISSN 0323-5408

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    Abstract

    One strain of Amycolatopsis sp. BCA-696, a rare genus of actinomycete, demonstrated previously for its plant growth-promotion traits in chickpea and sorghum, was tested for its antagonistic potential against Macrophomina phaseolina (the causal agent of charcoal rot disease of sorghum) by dual culture assay, metabolite production assay and in greenhouse and field screens. In the dual culture and metabolite production assays, BCA-696 inhibited the growth of M. phaseolina. When BCA-696 was tested for its antagonistic activity under greenhouse and field conditions (two seasons) against charcoal rot of sorghum by tooth pick method of inoculation, it significantly reduced the disease. Scanning electron microscope analysis revealed that the xylem and phloem tissues of the BCA-696 treated stem samples were intact compared to that of disease control plants. This study indicates that the selected Amycolatopsis sp. BCA-696 has the potential to manage charcoal rot of sorghum.

    Item Type: Article
    Divisions: Research Program : Asia
    CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
    Uncontrolled Keywords: Charcoal Rot, Sorghum, Amycolatopsis sp., Biocontrol, Greenhouse, Field Conditions
    Subjects: Others > Cereals
    Others > Plant Protection
    Others > Integrated Crop Management (ICM)
    Mandate crops > Sorghum
    Others > Plant Growth
    Others > Plant Disease
    Depositing User: Mr Arun S
    Date Deposited: 17 Oct 2019 04:10
    Last Modified: 18 Oct 2019 10:40
    URI: http://oar.icrisat.org/id/eprint/11323
    Official URL: https://doi.org/10.1080/03235408.2018.1553472
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: This work has been undertaken as part of the CGIAR Research Program on Grain Legumes Dry Land Cereals. ICRISAT is a member of CGIAR Consortium. We thank Dr M Lakshman, Associate Professor, Ruska Lab, College of Veterinary Science, Rajendranagar, Hyderabad, for SEM analysis. We also thank Mr PVS Prasad for his significant contribution in the laboratory, greenhouse and field studies.
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