Screening for anti-fungal proteins in pearl millet inbreds active against Pyricularia grisea

Marri, S and Sharma, R and Govindaraj, M (2018) Screening for anti-fungal proteins in pearl millet inbreds active against Pyricularia grisea. In: 1st National Genetics Congress on Genetics for Sustainable Food, Health and Nutrition Security, December 14-16, 2018, ICAR-lndian Agricultural Research Institute, New Delhi, India.

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Pearl millet is a commonly grown nutri-cereals in the semi-arid regions of India and Africa. Productivity of this crop is challenged by many biotic stress and blast (Pyricularia grisea) has emerged as a new threat. Recurrent chemical protection is expensive and not sustainable approach for farmers. Therefore, identification and utilization of host plant resistance is very appropriate. Present study screened 20 inbreds crude proteins for anti-fungal activity against blast isolates collected from Patancheru (Pg45), Jaipur (Pg138) and Aurangabad (Pg186) using oatmeal agar plates (qualitative) and microspectrophotometric assays (quantitative). There was significant reduction in growth rate of fungal colony of highly virulent isolate Pg 138 (9-57%; P<0.01)on oatmeal agar plates at fixed time intervals against Pg138, while no significant differences were observed for Pg 45 and Pg 186.Further, inhibition of fungal spore germination and initial growth (48h) of these isolates was monitored quantitatively (595nm) in the presence of each crude protein extract for 48h. As a result, high reduction in fungal growth was observed for Pg138 (64-87%) followed by Pg186 (21-61%) and Pg45 (2- 31%). Total protein in the tested lines varied 10-15%. Results showed that these protein extracts were effective (9-50%) against cysteine protease, papain commercially sourced from papaya that indicates millet protein extracts contain cysteine protease inhibitors and not a trypsin inhibitor. The result suggest that these PI may be used as potential alternative to anti-fungal agents, and merits further studies contributing to resistance breeding.

Item Type: Conference or Workshop Item (Paper)
Divisions: Research Program : Asia
Uncontrolled Keywords: pearl millet, biotic stress, blast isolates
Subjects: Mandate crops > Millets > Pearl Millet
Depositing User: Mr Arun S
Date Deposited: 04 Sep 2019 09:30
Last Modified: 04 Sep 2019 09:30
Acknowledgement: UNSPECIFIED
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