Identification of genomic regions and diagnostic markers for resistance to aflatoxin contamination in peanut (Arachis hypogaea L.)

Yu, B and Huai, D and Huang, L and Kang, Y and Ren, X and Chen, Y and Zhou, X and Luo, H and Liu, N and Chen, W and Lei, Y and Pandey, M K and Sudini, H and Varshney, R K and Liao, B and Jiang, H (2019) Identification of genomic regions and diagnostic markers for resistance to aflatoxin contamination in peanut (Arachis hypogaea L.). BMC Genetics (TSI), 20 (1). pp. 1-13. ISSN 1471-2156

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Abstract

Background Aflatoxin contamination caused by Aspergillus flavus is a major constraint to peanut industry worldwide due to its toxicological effects to human and animals. Developing peanut varieties with resistance to seed infection and/or aflatoxin accumulation is the most effective and economic strategy for reducing aflatoxin risk in food chain. Breeding for resistance to aflatoxin in peanut is a challenging task for breeders because the genetic basis is still poorly understood. To identify the quantitative trait loci (QTLs) for resistance to aflatoxin contamination in peanut, a recombinant inbred line (RIL) population was developed from crossing Zhonghua 10 (susceptible) with ICG 12625 (resistant). The percent seed infection index (PSII), the contents of aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) of RILs were evaluated by a laboratory kernel inoculation assay. Results Two QTLs were identified for PSII including one major QTL with 11.32–13.00% phenotypic variance explained (PVE). A total of 12 QTLs for aflatoxin accumulation were detected by unconditional analysis, and four of them (qAFB1A07 and qAFB1B06.1 for AFB1, qAFB2A07 and qAFB2B06 for AFB2) exhibited major and stable effects across multiple environments with 9.32–21.02% PVE. Furthermore, not only qAFB1A07 and qAFB2A07 were co-localized in the same genetic interval on LG A07, but qAFB1B06.1 was also co-localized with qAFB2B06 on LG B06. Conditional QTL mapping also confirmed that there was a strong interaction between resistance to AFB1 and AFB2 accumulation. Genotyping of RILs revealed that qAFB1A07 and qAFB1B06.1 interacted additively to improve the resistance to both AFB1 and AFB2 accumulation. Additionally, validation of the two markers was performed in diversified germplasm collection and four accessions with resistance to aflatoxin accumulation were identified. Conclusions Single major QTL for resistance to PSII and two important co-localized intervals associated with major QTLs for resistance to AFB1 and AFB2. Combination of these intervals could improve the resistance to aflatoxin accumulation in peanut. SSR markers linked to these intervals were identified and validated. The identified QTLs and associated markers exhibit potential to be applied in improvement of resistance to aflatoxin contamination.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Aflatoxin contamination, Aspergillus flavus, peanut, groundnut, quantitative trait loci, QTLs, Resistance, Aflatoxin, diagnostic marker, QTL Mapping, phenotypic evaluation, gene discovery, genetics, gene mapping, molecular breeding, aflatoxin resistance
Subjects:	Mandate crops > Groundnut Others > Genetics and Genomics Others > Aflatoxins
Depositing User:	Mr Ramesh K
Date Deposited:	04 Jul 2019 09:24
Last Modified:	04 Jul 2019 09:24
URI:	http://oar.icrisat.org/id/eprint/11140
Official URL:	https://doi.org/10.1186/s12863-019-0734-z
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This research was supported by the National Natural Science Foundation of China (31371662 and 31461143022), Fundamental Research Funds for Central Non-profit Scientific Institution (Y2018PT59) and China Agriculture Research System (CARS-14). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Links:	Google Scholar

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