InDel markers: An extended marker resource for molecular breeding in chickpea

Jain, A and Roorkiwal, M and Kale, S and Garg, V and Yadala, R and Varshney, R K (2019) InDel markers: An extended marker resource for molecular breeding in chickpea. PLoS ONE (TSI), 14 (3). pp. 1-13. ISSN 1932-6203

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Abstract

Chickpea is one of the most important food legumes that holds the key to meet rising global food and nutritional demand. In order to deploy molecular breeding approaches in crop improvement programs, user friendly and cost effective marker resources remain prerequisite. The advent of next generation sequencing (NGS) technology has resulted in the generation of several thousands of markers as part of several large scale genome sequencing and re-sequencing initiatives. Very recently, PCR based Insertion-deletions (InDels) are becoming a popular gel based genotyping solution because of their co-dominant, inexpensive, and highly polymorphic nature. With an objective to expand marker resources for genomics assisted breeding (GAB) in chickpea, whole genome re-sequencing data generated on five parental lines of one interspecific (ICC 4958 × PI 489777) and two intra-specific (ICC 283 × ICC 8261 and ICC 4958 × ICC 1882) mapping populations, were used for identification of InDels. A total of 231,658 InDels were identified using Dindel software with default parameters. Further, a total of 8,307 InDels with ≥20 bp size were selected for development of gel based markers, of which primers could be designed for 7,523 (90.56%) markers. On average, markers appeared at a frequency of 1,038 InDels/LG with a maximum number of markers on CaLG04 (1,952 InDels) and minimum on CaLG08 (360 InDels). In order to validate these InDels, a total of 423 primer pairs were randomly selected and tested on the selected parental lines. A high amplification rate of 80% was observed ranging from 46.06 to 58.01% polymorphism rate across parents on 3% agarose gel. This study clearly reflects the usefulness of available sequence data for the development of genome-wide InDels in chickpea that can further contribute and accelerate a wide range of genetic and molecular breeding activities in chickpea.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	Chickpea, Genomics, InDels, sequencing data, genome-wide markers, InDel markers, genetic studies, chickpea improvement, chickpea accessions
Subjects:	Others > Crop Improvement Others > Molecular Biology Others > Plant Breeding Mandate crops > Chickpea Others > Genetics and Genomics
Depositing User:	Mr Ramesh K
Date Deposited:	21 Jun 2019 05:51
Last Modified:	21 Jun 2019 05:51
URI:	http://oar.icrisat.org/id/eprint/11128
Official URL:	https://doi.org/10.1371/journal.pone.0213999
Projects:	Tropical Legumes III
Funders:	Bill & Melinda Gates Foundation (Tropical Legumes III), and Department of Biotechnology, Govt of India
Acknowledgement:	The authors are thankful to Bill & Melinda Gates Foundation (Tropical Legumes III), and Department of Biotechnology, Govt of India under IABF scheme for financial assistance. The work reported in this article was undertaken as a part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of the CGIAR.
Links:	Google Scholar

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