Sequencing Analysis of Genetic Loci for Resistance for Late Leaf Spot and Rust in Peanut (Arachis hypogaea L.)

Shirasawa, K and Bhat, R S and Khedikar, Y P and Sujay, V and Kolekar, R M and Yeri, S B and Sukruth, M and Cholin, S and Asha, B and Pandey, M K and Varshney, R K and Gowda, M V C (2018) Sequencing Analysis of Genetic Loci for Resistance for Late Leaf Spot and Rust in Peanut (Arachis hypogaea L.). Frontiers in Plant Science (TSI), 9 (1727). pp. 1-8. ISSN 1664-462X

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Abstract

The aim of this study was to identify candidate resistance genes for late leaf spot (LLS) and rust diseases in peanut (Arachis hypogaea L.). We used a double-digest restriction-site associated DNA sequencing (ddRAD-Seq) technique based on next-generation sequencing (NGS) for genotyping analysis across the recombinant inbred lines (RILs) derived from a cross between a susceptible line, TAG 24, and a resistant line, GPBD 4. A total of 171 SNPs from the ddRAD-Seq together with 282 markers published in the previous studies were mapped on a genetic map covering 1510.1 cM. Subsequent quantitative trait locus (QTL) analysis revealed major genetic loci for LLS and rust resistance on chromosomes A02 and A03, respectively. Heterogeneous inbred family-derived near isogenic lines and the pedigree of the resistant gene donor, A. cardenasii Krapov. & W.C. Greg., including the resistant derivatives of ICGV 86855 and VG 9514 as well as GPBD 4, were employed for whole-genome resequencing analysis. The results indicated the QTL candidates for LLS and rust resistance were located in 1.4- and 2.7-Mb genome regions on A02 and A03, respectively. In these regions, four and six resistance-related genes with deleterious mutations were selected as candidates for LLS and rust resistance, respectively. These delimited genomic regions may be beneficial in breeding programs aimed at improving disease resistance and enhancing peanut productivity.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: UNSPECIFIED
Uncontrolled Keywords: late leaf spot and rust diseases, peanut, quantitative trait locus, restriction-site associated DNA sequencing, whole-genome resequencing analysis
Subjects: Mandate crops > Groundnut
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 06 Dec 2018 09:51
Last Modified: 06 Dec 2018 09:51
URI: http://oar.icrisat.org/id/eprint/10999
Official URL: http://dx.doi.org/10.3389/fpls.2018.01727
Projects: UNSPECIFIED
Funders: DST-JSPS Bilateral Program and the Kazusa DNA Research Institute Foundation
Acknowledgement: We are grateful to S. Sasamoto and C. Mimani (Kazusa DNA Research Institute) for technical assistance.
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