Response to early drought stress and identification of QTLs controlling biomass production under drought in pearl millet

Debieu, M and Sine, B and Passot, S and Grondin, A and Akata, E and Gangashetty, P I and Vadez, V and Gantet, P and Fonceka, D and Cournac, L and Hash, C T and Kane, N A and Vigouroux, Y and Laplaze, L (2018) Response to early drought stress and identification of QTLs controlling biomass production under drought in pearl millet. PLoS One (TSI). pp. 1-19. ISSN 2191-5547

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Abstract

Pearl millet plays a major role in food security in arid and semi-arid areas of Africa and India. However, it lags behind the other cereal crops in terms of genetic improvement. The recent sequencing of its genome opens the way to the use of modern genomic tools for breeding. Our study aimed at identifying genetic components involved in early drought stress tolerance as a first step toward the development of improved pearl millet varieties or hybrids. A panel of 188 inbred lines from West Africa was phenotyped under early drought stress and well-irrigated conditions. We found a strong impact of drought stress on yield components. This impact was variable between inbred lines. We then performed an association analysis with a total of 392,493 SNPs identified using Genotyping-by-Sequencing (GBS). Correcting for genetic relatedness, genome wide association study identified QTLs for biomass production in early drought stress conditions and for stay-green trait. In particular, genes involved in the sirohaem and wax biosynthesis pathways were found to co-locate with association loci. Our results open the way to use genomic selection to breed pearl millet lines with improved yield under drought stress.

Item Type: Article
Divisions: Research Program : West & Central Africa
CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords: Drought stress, identification of QTLs, biomass production, drought, pearl millet, Genome wide association study (GWAS), QTLs
Subjects: Others > Drought Tolerance
Mandate crops > Millets > Pearl Millet
Others > Drylands Agriculture
Others > Drought
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 30 Oct 2018 09:51
Last Modified: 30 Oct 2018 10:26
URI: http://oar.icrisat.org/id/eprint/10916
Official URL: https://doi.org/10.1371/journal.pone.0201635
Projects: UNSPECIFIED
Funders: IRD, the French Ministry for Research and Higher Education (PhD grant to SP) and the NewPearl grant in the frame of the CERES initiative by the Agropolis Fondation
Acknowledgement: This work was supported by the IRD, the French Ministry for Research and Higher Education (PhD grant to SP) and the NewPearl grant in the frame of the CERES initiative by the Agropolis Fondation (N° AF 1301–015 to LL, as part of the "Investissement d'avenir" ANR-l0-LABX-0001-0l) under the frame of I-SITE MUSE (ANR-16-IDEX-0006) and by the Fondazione Cariplo (N° FC 2013–0891).
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