Proteomic and transcriptomic approaches to identify resistance and susceptibility related proteins in contrasting rice genotypes infected with fungal pathogen Rhizoctonia solani

Prathi, N B and Palit, P and Madhu, P and Ramesh, M and Laha, G S and Balachandran, S M and Madhav, M S and Sundaram, R M and Mangrauthia, S K (2018) Proteomic and transcriptomic approaches to identify resistance and susceptibility related proteins in contrasting rice genotypes infected with fungal pathogen Rhizoctonia solani. Plant Physiology and Biochemistry (TSI), 130. pp. 258-266. ISSN 09819428

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Abstract

The devastating sheath blight disease caused by Rhizoctonia solani Kuhn (teleomorph: Thanatephorus cucumeris) causes major yield loss in most rice growing regions of the world. In this study, two moderately tolerant and four susceptible genotypes of rice were selected for R. solani induced proteome analysis using two-dimensional polyacrylamide gel electrophoresis. Forty five differentially expressed proteins (DEPs) were identified and analyzed by Mass Spectrometry. Based on their functions, these proteins were classified into different groups, viz., photosynthesis, resistance and pathogenesis, stress, cell wall metabolism and cytoskeleton development associated proteins, and hypothetical or uncharacterized proteins. Expression of 14 genes encoding DEPs was analyzed by quantitative PCR which showed consistency in transcripts and genes expression pattern. Furthermore, the expression of 16 other genes involved in diverse biological functions was analyzed. Up-regulation of these genes in the tolerant genotype Pankaj during sheath blight disease suggested efficient genetic regulation of this cultivar under stress. Also, expression analysis of conserved microRNAs (miRNAs) and their target genes revealed important role of miRNAs in post-transcriptional gene regulation during development of rice sheath blight disease. Genome-wide discovery of miRNAs and further characterization of DEPs and genes will help in better understanding of the molecular events during sheath blight disease development in rice.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: UNSPECIFIED
Uncontrolled Keywords: 2D-gel electrophoresis, Sheath blight, Oryza sativa, Gene expression, microRNA, Rice, Blight disease
Subjects: Others > Rice
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 22 Oct 2018 10:03
Last Modified: 23 Oct 2018 09:57
URI: http://oar.icrisat.org/id/eprint/10905
Official URL: http://dx.doi.org/10.1016/j.plaphy.2018.07.012
Projects: UNSPECIFIED
Funders: Financial support received from DBT grant (BT/PR6466/COE/34/ 16/2012) is acknowledged
Acknowledgement: Authors are thankful to the Director, ICAR-IIRR, for his kind support. Financial support received from DBT grant (BT/PR6466/COE/34/ 16/2012) is acknowledged. We are highly thankful to Dr. K. Veluthambi (Madurai Kamaraj University) for his valuable suggestions during the manuscript preparation. The facility of 2D gel imaging and analysis was kindly provided by Dr. V. Dinesh Kumar (ICAR-IIOR). We are also grateful to anonymous reviewers for their constructive suggestions to improve this manuscript.
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