QTL mapping and validation of fertility restoration in West African sorghum A1 cytoplasm and identification of a potential causative mutation for Rf2

Kante, M and Rattunde, H F W and Nebie, B and Weltzien, E and Haussmann, B I G and Leiser, W L (2018) QTL mapping and validation of fertility restoration in West African sorghum A1 cytoplasm and identification of a potential causative mutation for Rf2. Theoretical and Applied Genetics (TSI). pp. 1-16. ISSN 0040-5752

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Abstract

Abstract Key message Major A 1 cytoplasm fertility restoration loci, Rf 2 and Rf 5 , were found in the West African sorghum. A potential causative mutation for Rf 2 was identified. KASP markers were validated on independent material. Abstract To accelerate the identification and development of hybrid parental lines in West African (WA) sorghum, this study aimed to understand the genetics underlying the fertility restoration (Rf) in WA A1 cytoplasmic male sterility system and to develop markers for a routine use in WA breeding programs. We genotyped by sequencing three F2 populations to map the Rf quantitative trait loci (QTL), validated the molecular KASP markers developed from those QTL in two F2:3 populations, and assessed the most promising markers on a set of 95 R- and B-lines from WA breeding programs. Seven QTL were found across the three F2 populations. On chromosome SBI-05, we found a major fertility restorer locus (Rf5) for two populations with the same male parent, explaining 19 and 14% of the phenotypic variation in either population. Minor QTL were detected in these two populations on chromosomes SBI-02, SBI-03, SBI-04 and SBI-10. In the third population, we identified one major fertility restorer locus on chromosome SBI-02, Rf2, explaining 31% of the phenotypic variation. Pentatricopeptide repeat genes in the Rf2 QTL region were sequenced, and we detected in Sobic.002G057050 a missense mutation in the first exon, explaining 81% of the phenotypic variation in a F2:3 population and clearly separating B- from R-lines. The KASP marker developed from this mutation stands as a promising tool for routine use in WA breeding programs.

Item Type: Article
Divisions: Research Program : West & Central Africa
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Sorghum bicolor, Fertility restoration, QTL mapping, West Africa, Gene sequencing
Subjects: Mandate crops > Sorghum
Others > Genetics and Genomics
Others > West Africa
Depositing User: Mr Ramesh K
Date Deposited: 30 Aug 2018 10:43
Last Modified: 30 Aug 2018 10:43
URI: http://oar.icrisat.org/id/eprint/10850
Official URL: http://dx.doi.org/10.1007/s00122-018-3161-z
Projects: BMZ-Heterosis Project
Funders: German Ministry for Economic Cooperation and Development (BMZ)
Acknowledgement: The authors thank the technical staff of the sorghum breeding program in ICRISAT-Mali and specifically Abdoul Karim Touré and Marthe Dembélé for their contribution in phenotyping. We also acknowledge the support of the lab personnel at the State Plant Breeding Institute of the University of Hohenheim, particularly Stefanie Laucher, for candidate gene sequencing and marker creation. We would like to acknowledge the funding by the German Ministry for Economic Cooperation and Development (BMZ) via the BMZ-Heterosis Project (ICRISAT Project 13.1432.7-001.00), and also the supplemental financial support from the McKnight Foundation Collaborative Crop Research Program via discretionary research funds provided to B.I.G. Haussmann. This work was undertaken as a part of the CGIAR Research Program on Dryland Cereals.
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