Biochemical diversity evaluation in chickpea accessions employing mini-core collection

Bhagyawant, S S and Gautam, A K and Narvekar, D T and Gupta, N and Bhadkaria, A and Srivastava, N and Upadhyaya, H D (2018) Biochemical diversity evaluation in chickpea accessions employing mini-core collection. Physiology and Molecular Biology of Plants (TSI). pp. 1-19. ISSN 0971-5894

PDF - Published Version Restricted to ICRISAT users only Download (2MB) | Request a copy

Abstract

The seeds of chickpea provide an exceptional source of dietary proteins and is one of the important legumes in both developed and developing countries over the world. The available germplasm of cultivated chickpea is deficient in desired biochemical signatures. To identify new sources of variations for breeding, reduced subsets of germplasm such as mini-core collection can be explored as an effective resource. In the present investigation, mini-core collections consisting of 215 accessions of chickpea were extensively evaluated for tapping biochemical diversity. Analysis included ten biochemical parameters comprising total protein, total free amino acids, phytic acid, tannin, total phenolics, total flavonoids, lectin, DPPH radical scavenging activity, in vitro digestibility of protein and starch. The spectrum of diversity was documented for total protein (4.60–33.90%), total free amino acids (0.092–9.33 mg/g), phytic acid (0.009–4.06 mg/g), tannin (0.232–189.63 mg/g), total phenolics (0.15–0.81 mg/g), total flavonoids (0.04–1.57 mg/g), lectin (0.07–330.32 HU/mg), DPPH radical scavenging activity (26.74–49.11%), in vitro protein digestibility (59.45–76.22%) and in vitro starch digestibility (45.63–298.39 mg of maltose/g). The principal component analysis revealed association of chickpea higher protein content to the lower level of total phenolics and flavonoid contents. The dendrogram obtained by unweighted pair group method using arithmetic average cluster analysis grouped the chickpea accessions into two major clusters. This is the first comprehensive report on biochemical diversity analysed in the mini-core chickpea accessions. The ultimate purpose of conducting such studies was to deliver information on nutritional characteristics for effective breeding programmes. Depending on the objectives of the breeding aforesaid accessions could be employed as a parent.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Chickpea, Minicore collection, genetic diversity, In vitro protein digestibility, lectin, DPPH, chickpea accessions, biochemical diversity, mini core chickpea accessions, breeding programme, germplasm, biofortification strategies
Subjects:	Mandate crops > Chickpea Others > Genetics and Genomics Others > Germplasm
Depositing User:	Mr Ramesh K
Date Deposited:	06 Aug 2018 08:55
Last Modified:	06 Aug 2018 08:55
URI:	http://oar.icrisat.org/id/eprint/10822
Official URL:	http://dx.doi.org/10.1007/s12298-018-0579-3
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Ajay Kumar Gautam is grateful to University of Grants Commission (UGC), New Delhi for providing research fellowship under major research project (F.No. 40-154/2011 (SR). Authors are also grateful to ICRISAT, Hyderabad, (A.P.) India for providing the seed samples. Dr. Sushma Tiwari, Scientist, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior is thankfully acknowledged for providing statistical software support.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item