Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought stress tolerance

    Somanaboina, A K and Narasu, L N and Varshney, R K and Kavi Kishor, P B (2017) Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought stress tolerance. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.

    [img]
    Preview
    		 PDF (Poster Abstract) - Published Version
    Download (152kB) | Preview

    Abstract

    Tomato (Solanum lycopersicum L.) is the second most produced and consumed vegetable, next to potato. Pusa early dwarf (PED) variety of tomato is sensitive to salinity and drought which reduces the yield and fruit quality in tomato. In the present study, tomato transgenics were developed with osmotin-like protein (OLP) and chitinase (Chi11) genes via in vitro and in planta transformation methods. Transgene integration and transcript levels were confirmed by multiplex PCR, DNA blot, and multiplex reverse transcriptase PCR. Homozygous T2 transgenics, when evaluated for salt and drought stress, showed enhanced tolerance compared to wild-type plants. Transgenics showed enhanced root biomass under normal conditions. Transformants also displayed higher proline content, K+, relative water content, chlorophyll fluorescence, total biomass, vascular conductivity, and fruit yield compared to the wild-type plants under stress conditions. Co-immunoprecipitation revealed that Chi11 co-expresses with phosphofructokinase2 (PFK2), which may play a role in enhanced root biomass. qPCR analysis resulted in higher transcript levels of OLP, Chi11, and PFK2 in transgenics as compared to the wild-type plants.

    Item Type: Conference or Workshop Item (Paper)
    Divisions: Research Program : Genetic Gains
    CRP: UNSPECIFIED
    Uncontrolled Keywords: transgenic tomato, drought stress tolerance
    Subjects: Others > Abiotic Stress
    Others > Genetics and Genomics
    Depositing User: Mr Ramesh K
    Date Deposited: 13 Jun 2018 11:27
    Last Modified: 28 Aug 2019 06:25
    URI: http://oar.icrisat.org/id/eprint/10740
    Acknowledgement: UNSPECIFIED
    Links:
    View Statistics

    Actions (login required)

    View Item View Item
    OAR@ICRISAT is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.