Deciphering the tri-dimensional effect of endophytic Streptomyces sp. on chickpea for plant growth promotion, helper effect with Mesorhizobium ciceri and host-plant resistance induction against Botrytis cinerea

Vijayabharathi, R and Gopalakrishnan, S and Sathya, A and Srinivas, V and Sharma, M (2018) Deciphering the tri-dimensional effect of endophytic Streptomyces sp. on chickpea for plant growth promotion, helper effect with Mesorhizobium ciceri and host-plant resistance induction against Botrytis cinerea. Microbial Pathogenesis (TSI), 122. pp. 98-107. ISSN 08824010

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Abstract

A total of 219 endophytic actinobacteria, isolated from roots, stems and leaves of chickpea, were characterized for antagonistic potential against Botrytis cinerea, causal organism of Botrytis grey mold (BGM) disease, in chickpea. Among them, three most potential endophytes, AUR2, AUR4 and ARR4 were further characterized for their plant growth-promoting (PGP) and nodulating potentials and host-plant resistance against B. cinerea, in chickpea. The sequences of 16 S rDNA gene of the three endophytes were matched with Streptomyces but different species. In planta, the isolate AUR4 alone was able to significantly enhance PGP traits including seed numbers (11.8 vs. 9.8/Plant), seed weight (8 vs. 6.8 g/Plant), pod numbers (13.6 vs. 11.5/Plant), pod weight (9.3 vs. 7.5 g/Plant) and biomass (10.9 vs. 8 g/Plant) over the un-inoculated control in chickpea genotype JG11. Interestingly, consortium of the selected endophytes, AUR2, AUR4 and ARR4 were found less effective than single inoculation. Co-inoculation of the selected endophytes with Mesorhizobium ciceri significantly enhanced nodulation and nitrogenase activity in five chickpea genotypes including ICCV2, ICCV10, ICC4958, Annigeri and JG11 over the un-inoculated control. The selected endophytes showed antagonistic potential in planta by significant reduction of disease incidence (28─52%) in both single inoculation and consortium treatments over the un-inoculated control across the genotypes ICC4954 (susceptible), ICCV05530 (moderately resistant) and JG11 (unknown resistance). Further, antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase, phenylalanine ammonia-lyase and polyphenol oxidase and phenolics were found induced in the leaves of chickpea inoculated with selected endophytes over un-inoculated control. Principal component analysis revealed that, the antioxidant enzymes and phenolics were found in the magnitude of ICC4954 < JG11 < ICCV05530 which correlates with their resistance level. The selected endophytes enhanced the plant growth and also host plant resistance against BGM in chickpea.

Item Type: Article
Divisions: Research Program : Asia
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Endophytes, Chickpea, Streptomyces, Botrytis cinerea, Host plant resistance, Antioxidants, Nodulation, chickpea genotypes
Subjects: Others > Crop Improvement
Mandate crops > Chickpea
Others > Genetics and Genomics
Others > Plant Growth
Depositing User: Mr Ramesh K
Date Deposited: 13 Jun 2018 06:08
Last Modified: 13 Jun 2018 06:11
URI: http://oar.icrisat.org/id/eprint/10716
Official URL: http://dx.doi.org/10.1016/j.micpath.2018.06.019
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This study is supported by Young Scientist scheme of SERB, DST, India through SB/YS/LS-345/2013 and author RV is grateful to this. This work was undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. We thank Mr K Sivasakthi, Crop Physiology, ICRISAT for statistical assistance, Dr G Senthilraja Centre of Excellence in Millets, Tamil Nadu Agricultural University, Tiruvannamalai in AUDPC calculation. Technical assistance of Mr Rameshwar for BGM experiments is also acknowledged. We also thank our lab members M/s PVS Prasad and A Jabbar for technical assistance.
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