Fine mapping of the linkage group 2 drought tolerance QTL in pearl millet [Pennisetum glaucum (L.) R. Br.]

Srivastava, R K and Vadez, V and Kholova, J and Lachagari, V B R and Mahendrakar, M D and Katiyar, P and Lekkala, S P and Praveen, M and Gupta, R and Blumwald, E (2017) Fine mapping of the linkage group 2 drought tolerance QTL in pearl millet [Pennisetum glaucum (L.) R. Br.]. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.

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Pearl millet is an important cereal crop capable of growing in semi-arid, arid and marginal regions of the world. Drought is a major abiotic constraint affecting pearl millet production globally. Previously identified and validated major linkage group 2 (LG2) drought tolerance (DT) QTL contributing to hybrid grain and stover yield potential to terminal drought stress is being fine mapped. The fine mapping population was generated by crossing two near-isogenic lines (NILs) differing for the LG2 DT QTL. A modified double digest restriction site associated DNA (ddRAD) technique using SphI and MluCI enzyme combination was employed. A total of 290 out of 6,067 F2 mapping population of the cross (H77/833-2-P10 × ICMR 01029-P10), and parents were genotyped on Illumina HiSeq2500. Out of 52,028 SNPs that were identified from a total of 888.85 million reads at a read depth of 10 in the mapping population, a total of 6,821 SNPs were used for mapping. The genotypic data of these SNPs were used in combination with that of seven SSRs that had known linkage relationship with LG2 DT QTL interval. Linkage map was constructed using QTL IciMapping 4.1 software at a LOD threshold of 3.0. A total of 189 SNPs anchored to seven SSRs were mapped to the LG2 DT QTL. The length of linkage group (LG) was 639.72 cM (Haldane units) with an average inter-marker distance of 6.73 cM. In order to refine the fine mapping process, ddRAD technique is being further employed for genotyping rest of the fine mapping population.

Item Type: Conference or Workshop Item (Paper)
Divisions: Research Program : Genetic Gains
Research Program : Innovation Systems for the Drylands (ISD)
Uncontrolled Keywords: Fine mapping, drought tolerance, QTL, pearl millet
Subjects: Others > Abiotic Stress
Mandate crops > Millets > Pearl Millet
Depositing User: Mr Ramesh K
Date Deposited: 12 Jun 2018 09:29
Last Modified: 12 Jun 2018 09:29
Acknowledgement: UNSPECIFIED
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