Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis

Alex, N and Cecilia, M and Mathew, N and Asunta, M and Henry, O and Wilton, M (2018) Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis. African Journal of Biotechnology, 17 (21). pp. 660-667. ISSN 1684-5315

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Abstract

In the present study, an efficient protocol for somatic embryogenesis and plant regeneration was established in six finger millet varieties (GBK-043137, GBK-043128, GBK-043124, GBK-043122, GBK- 043094 and GBK-043050). Shoot tips from 3 days in vitro grown plants were inoculated on MS supplemented with various concentrations and combinations of α-naphthaleneacetic acid (NAA), 2,4- Dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and kinetin for callus induction and somatic embryogenesis. For shoot regeneration, somatic embryos were cultured on various concentrations of BAP, while root induction was done using different concentrations and combinations of NAA, kinetin, BAP and 2,4-D. Acclimatization of regenerated plants was tested using forest soil, cocopeat, manure, sand and fertilizer either singly or in combination. Best callus formation was achieved on 2.5 mg/l of 2,4-D and 1.5 mg/l BAP with a mean of 12.33±0.33 on variety GBK-043128 while shooting and rooting were best on 1.75 mg/l BAP with a mean of 25.07±0.64 and 1.0 BAP+0.25 NAA with a mean of 15.00±2.2, respectively. Best acclimatization was attained using soil, sand and fertilizer on GBK-043094. Plants regenerated were morphologically similar to in vivo plants with 97% survival rate. Moreover, they were fertile and able to set viable seeds. This efficient protocol has the potential for crop improvement and genomic studies.

Item Type: Article
Divisions: Research Program : East & Southern Africa
CRP: UNSPECIFIED
Uncontrolled Keywords: Finger millet, plant regeneration, shoot tips, somatic embryogenesis, transgenic technologies, plant growth, Kenya
Subjects: Mandate crops > Millets > Pearl Millet
Others > Plant Physiology
Others > Plant Growth
Others > East Africa
Depositing User: Mr Ramesh K
Date Deposited: 28 May 2018 10:20
Last Modified: 28 May 2018 10:20
URI: http://oar.icrisat.org/id/eprint/10685
Official URL: http://dx.doi.org/10.5897/AJB2018.16452
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We are grateful to Kenya Agricultural and Livestock Research Organization, Gene Bank, for providing the finger millet seed used. Support for this research was made possible through a capacity building competitive grant (RU/2016/DRRG/01/009) provided by Regional Universities Forum for Capacity Building in Agriculture (RUFORUM).
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