Influence of diazotrophic bacteria on nodulation, nitrogen fixation, growth promotion and yield traits in five cultivars of chickpea

Gopalakrishnan, S and Srinivas, V and Anil Kumar, V and Samineni, S and Rathore, A (2018) Influence of diazotrophic bacteria on nodulation, nitrogen fixation, growth promotion and yield traits in five cultivars of chickpea. Biocatalysis and Agricultural Biotechnology, 15. pp. 35-42. ISSN 1878-8181

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Abstract

Three bacteria, IC-59, IC-76A and IC-2002, isolated from the nodules of chickpea, were characterized for nodulation, nitrogen fixation, plant growth-promoting (PGP) and yield traits in five cultivars of chickpea such as BG256, RSG888, Subhra, K850 and ICCV2. All the bacteria produced cellulase, protease, β-1,3-glucanase, indole acetic acid, siderophore, hydro cyanic acid and 1-aminocyclopropane-1-carboxylate deaminase while none produced lipase and chitinase. The 16 S rDNA gene sequences of IC-59, IC-76A and IC-2002 were found to match closely with Rhizobium pusense, Paraburkholderia kururiensis and Stenotrophomonas maltophilia, respectively. The three bacteria nodulated all the cultivars of chickpea well, amplified nifH gene and fixed nitrogen. Under greenhouse conditions at 30 and 45 days after sowing, treatment of five cultivars of chickpea with bacterial cultures IC-59, IC-76A and IC-2002, enhanced the nodule number (up to 45%, 38% and 43%), nodule weight (up to 31%, 15% and 39%), shoot weight (11%, 16% and 14%) and root weight (37%, 48% and 62%), respectively, over the un-inoculated control. At crop maturity, IC-59, IC-76A and IC-2002 were found to enhance the shoot weight (16%, 40% and 26%), pod number (37%, 69% and 81%), pod weight (17%, 45% and 49%), seed number (21%, 31% and 39%) and seed weight (14%, 56% and 65%), respectively, over the un-inoculated control. Among the five cultivars, Subhra was found to enhance most of the PGP traits when treated with the three diazotrophic bacteria. It is concluded that the three diazotrophic bacteria could be potentially exploited for improving nodulation, nitrogen fixation, PGP and yields of chickpea.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	CGIAR Research Program on Grain Legumes
Uncontrolled Keywords:	Diazotrophic bacteria; Nodulation; Nitrogen fixation; Plant growth-promotion; Chickpea
Subjects:	Mandate crops > Chickpea Others > Plant Growth
Depositing User:	Mr Ramesh K
Date Deposited:	18 May 2018 06:28
Last Modified:	05 Jul 2019 09:14
URI:	http://oar.icrisat.org/id/eprint/10672
Official URL:	https://doi.org/10.1016/j.bcab.2018.05.006
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. We thank Mr PVSN Sharma and Ms. Danteswari, University of Hyderabad, Hyderabad for helping in amplification of nifH gene. We also thank Mr PVS Prasad for his significant contribution in the laboratory and green house studies.
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