Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor

Hima Kumari, P and Anil Kumar, S and Ramesh, K and Reddy, P S and Nagaraju, M and Bhanu Prakash, A and Shah, T and Henderson, A and Srivastava, R K and Rajasheker, G and Chitikineni, A and Varshney, R K and Rathnagiri, P and Lakshmi Narasu, M and Kavi Kishor, P B (2018) Genome-Wide Identification and Analysis of Arabidopsis Sodium Proton Antiporter (NHX) and Human Sodium Proton Exchanger (NHE) Homologs in Sorghum bicolor. Genes (TSI), 9 (5) (236). pp. 1-18. ISSN 2073-4425

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Abstract

Na+ transporters play an important role during salt stress and development. The present study is aimed at genome-wide identification, in silico analysis of sodium-proton antiporter (NHX) and sodium-proton exchanger (NHE)-type transporters in Sorghum bicolor and their expression patterns under varied abiotic stress conditions. In Sorghum, seven NHX and nine NHE homologs were identified. Amiloride (a known inhibitor of Na+/H+ exchanger activity) binding motif was noticed in both types of the transporters. Chromosome 2 was found to be a hotspot region with five sodium transporters. Phylogenetic analysis inferred six ortholog and three paralog groups. To gain an insight into functional divergence of SbNHX/NHE transporters, real-time gene expression was performed under salt, drought, heat, and cold stresses in embryo, root, stem, and leaf tissues. Expression patterns revealed that both SbNHXs and SbNHEs are responsive either to single or multiple abiotic stresses. The predicted protein–protein interaction networks revealed that only SbNHX7 is involved in the calcineurin B-like proteins (CBL)- CBL interacting protein kinases (CIPK) pathway. The study provides insights into the functional divergence of SbNHX/NHE transporter genes with tissue specific expressions in Sorghum under different abiotic stress conditions.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: UNSPECIFIED
Uncontrolled Keywords: Genome-wide screening; abiotic stress; amiloride; CBL-CIPK pathway; sodium-proton exchanger; sodium-proton antiporter; Sorghum bicolor
Subjects: Mandate crops > Sorghum
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 15 May 2018 04:37
Last Modified: 15 May 2018 04:37
URI: http://oar.icrisat.org/id/eprint/10660
Official URL: http://dx.doi.org/10.3390/genes9050236
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: Polavarapu B Kavi Kishor is thankful for the CSIR-Emeritus Scientist Fellowship through Grant 38(1325)/12/EMR-II) from the Council for Scientifics and Industrial Research, New Delhi. Palavalasa Hima Kumari is thankful to the University Grants Commission, New Delhi, for providing a Kothari postdoctoral fellowship (F.4-2/2006 (BSR)/BL/14-15/0392). Ashley Henderson and Katam Ramesh acknowledge the support from the National Science Foundation (Grant #1560049 Awarded to PI, Katam Ramesh).
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