Phenotypic Characteristics and Preliminary Symbiotic Effectiveness of Rhizobia Associated with Haricot Bean Growing in Diverse Locations of Southern Ethiopia

Wolde-meskel, E and Degefu, T and Gebo, B and Fikre, A and Amede, T and Ojiewo, C O (2018) Phenotypic Characteristics and Preliminary Symbiotic Effectiveness of Rhizobia Associated with Haricot Bean Growing in Diverse Locations of Southern Ethiopia. Ethiopian Journal of Crop Science, 6 (2). pp. 119-139. ISSN 2072-8506

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Abstract

Phenotypic characteristics of one hundred thirteen rhizobia nodulating haricot bean (Phaseolus vulgaris L.) growing on soils of nine different locations from southern Ethiopia were studied. Their tolerance to varying temperature, salinity, soil pH, heavy metals, antibiotics, their symbiotic effectiveness and cultural characteristics were determined. Eight reference species belonging to four different genera were also included in the analyses. The analyses allowed the description of a wide physiological diversity among tested isolates. Numerical analysis, based on numerical taxonomic approach, of the phenotypic characteristics, using Unweighted Pair Group Method with Average algorithm as implemented in NTSYspc21 software package, showed that, the tested isolates fell into five major diversity groups (designated as group I-V), when 82% level of relative similarity were used as cut-off point. Four strains were found to occupy a separate branch, thus designated as U (unclustered) group. While strains belonging to groups 1-IV were found to associate with recognized species belonging to Rhizobium, Ensifer and Mesorhizobium genus, the remaining test strains in cluster V and U were found to occupy distinct branches of their own on the dendrogram. Under laboratory condition, they were able to grow at pH ranging from 4 to 10.5; majority tolerated salt concentration (0.5-1%) and grew at a maximum temperature between 35 and 40 °C. The isolates were able to utilize a wide range of carbon and amino acid source and tolerated range of antibiotics and heavy metals. Based on the symbiotic effectiveness test, a number of potential isolates have been identified for inoculation trials.

Item Type: Article
Divisions: Research Program : East & Southern Africa
CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals
Uncontrolled Keywords: Haricot bean, Southern Ethiopia, Phenotypic characteristics, Rhizobium, Numerical taxonomy, Rhizobia
Subjects: Others > Soil
Others > Legume Crops
Others > Ethiopia
Depositing User: Mr Ramesh K
Date Deposited: 08 May 2018 07:28
Last Modified: 18 May 2018 09:03
URI: http://oar.icrisat.org/id/eprint/10639
Official URL:
Projects: Tropical Legumes III (TL III)
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
Links:

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