Validation of foreground microsatellite markers for introgression of shootfly (Atherigona soccata) resistant QTLs into elite sorghum varieties

Gorthy, S and Narasu, L and Ashok Kumar, A (2016) Validation of foreground microsatellite markers for introgression of shootfly (Atherigona soccata) resistant QTLs into elite sorghum varieties. International Journal of Scientific Research, 5 (12). pp. 663-667. ISSN 2277 - 8179

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Abstract

Sorghum is an important cereal and fodder crop which plays pivotal role in human nutrition. Important challenge that threatens the long term production of sorghum is shootfly. Shootfly is the major limiting factor in sorghum as it effects both production and productivity. Leaf glossiness, trichomes on the leaf surface, Ovipositional non-preference and seedling vigor are the major component traits governing shootfly resistance in sorghum. The quantitative trait loci's (QTLs) responsible for these traits are present on chromosome number SBI 01, SBI 05, SBI 07 and SBI 10 respectively. So, to provide ready to use markers for foreground selection in marker assisted breeding for sorghum we have used 20 recurrent parents and 9 donor parents for shootfly resistance. These simple sequence repeat (SSR) markers are validated for introgressing the QTL's into elite postrainy sorghum cultivars. The results reveal, out of 58 SSR markers, 33 markers showed distinct polymorphism among the donor and recurrent parents. For each QTL minimum of five markers from the QTL flanking region were polymorphic for all the parents and are used for foreground selection for the presence of QTLs.

Item Type: Article
Divisions: Research Program : Asia
CRP: UNSPECIFIED
Uncontrolled Keywords: Polymorphism, CTAB, Shootfly Sorghum, shoot fly, QTL, leaf glossiness, foreground selection
Subjects: Mandate crops > Sorghum
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 18 Apr 2018 05:09
Last Modified: 18 Apr 2018 05:10
URI: http://oar.icrisat.org/id/eprint/10590
Official URL:
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: The authors sincerely acknowledge the International Crops Research Institute for Semi Arid Tropics (ICRISAT) Patancheru, India for providing work space and Department of Science and Technology, New Delhi for providing funding for this research.
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