Towards Defining Heterotic Gene Pools in Pearl Millet [Pennisetum glaucum (L.) R. Br.]

Radhika Ramya, A and Lal Ahamed, M and Satyavathi, C T and Rathore, A and Katiyar, P and Bhasker Raj, A G and Kumar, S and Gupta, R and Mahendrakar, M D and Yadav, R S and Srivastava, R K (2018) Towards Defining Heterotic Gene Pools in Pearl Millet [Pennisetum glaucum (L.) R. Br.]. Frontiers in Plant Science (TSI), 8 (1934). pp. 1-11. ISSN 1664-462X

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Abstract

Pearl millet is a climate resilient crop and one of the most widely grown millets worldwide. Heterotic hybrid development is one of the principal breeding objectives in pearl millet. In a maiden attempt to identify heterotic groups for grain yield, a total of 343 hybrid parental [maintainer (B-) and restorer (R-)] lines were genotyped with 88 polymorphic SSR markers. The SSRs generated a total of 532 alleles with a mean value of 6.05 alleles per locus, mean gene diversity of 0.55, and an average PIC of 0.50. Out of 532 alleles, 443 (83.27%) alleles were contributed by B-lines with a mean of 5.03 alleles per locus. R-lines contributed 476 alleles (89.47%) with a mean of 5.41, while 441 (82.89%) alleles were shared commonly between B- and R-lines. The gene diversity was higher among R-lines (0.55) compared to B-lines (0.49). The unweighted neighbor-joining tree based on simple matching dissimilarity matrix obtained from SSR data clearly differentiated B- lines into 10 sub-clusters (B1 through B10), and R- lines into 11 sub-clusters (R1 through R11). A total of 99 hybrids (generated by crossing representative 9 B- and 11 R- lines) along with checks were evaluated in the hybrid trial. The 20 parents were evaluated in the line trial. Both the trials were evaluated in three environments. Based on per se performance, high sca effects and standard heterosis, F1s generated from crosses between representatives of groups B10R5, B3R5, B3R6, B4UD, B5R11, B2R4, and B9R9 had high specific combining ability for grain yield compared to rest of the crosses. These groups may represent putative heterotic gene pools in pearl millet.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Pearl millet, Heterotic hybrid development, grain yield, B- (maintainer) lines, R- (restorer) lines, SSR (simple sequence repeat) markers, gene diversity, PIC (polymorphism information content), heterotic groups, heterotic gene pools, heterotic hybrids
Subjects: Mandate crops > Millets > Pearl Millet
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 10 Apr 2018 05:33
Last Modified: 11 Apr 2018 07:42
URI: http://oar.icrisat.org/id/eprint/10542
Official URL: http://dx.doi.org/10.3389/fpls.2017.01934
Projects: UNSPECIFIED
Funders: S.M. Sehgal Foundation Endowment Fund
Acknowledgement: The research work was financially supported by the S.M. Sehgal Foundation Endowment Fund, ICRISAT. The authors are thankful to the authorities of ANGRAU for providing facilities to carry out field evaluations. This work has been published as part of the CGIAR Research Program on Dryland Cereals.
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