Imidacloprid impedes mitochondrial function and induces oxidative stress in cotton bollworm, Helicoverpa armigera larvae (Hubner: Noctuidae)

Nareshkumar, B and Akbar, S M and Sharma, H C and Jayalakshmi, S K and Sreeramulu, K (2018) Imidacloprid impedes mitochondrial function and induces oxidative stress in cotton bollworm, Helicoverpa armigera larvae (Hubner: Noctuidae). Journal of Bioenergetics and Biomembranes (TSI), 50 (1). pp. 21-32. ISSN 0145-479X

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Abstract

Neonicotinoids have high agonistic affinity to insect nicotinic acetylcholine receptors (nAChR) and are frequently used as insecticides against most devastating lepidopteran insect pests. Imidacloprid influenced dose-dependent decline in the state III and IV respiration, respiration control index (RCI), and P/O ratios, in vitro and in vivo. The bioassay indicated its LD50 value to be 531.24 μM. The insecticide exhibited a dose-dependent inhibition on F0F1-ATPase and complex IV activity. At 600 μM, the insecticide inhibited 83.62 and 27.13% of F0F1-ATPase and complex IV activity, respectively, and induced the release of 0.26 nmoles/min/mg protein of cytochrome c. A significant dose- and time-dependent increase in oxidative stress was observed; at 600 μM, the insecticide correspondingly induced lipid peroxidation, LDH activity, and accumulation of H2O2 content by 83.33, 31.51 and 223.66%. The stress was the maximum at 48 h of insecticide treatment (91.58, 35.28, and 189.80%, respectively). In contrast, catalase and superoxide dismutase were reduced in a dose- and time-dependent manner in imidacloprid-fed larvae. The results therefore suggest that imidacloprid impedes mitochondrial function and induces oxidative stress in H. armigera, which contributes to reduced growth of the larvae along with its neurotoxic effect.

Item Type: Article
Divisions: Research Program : Asia
CRP: UNSPECIFIED
Uncontrolled Keywords: Mitochondria, Oxidative phosphorylation, Oxidative stress, Imidacloprid, Neonicotinoids, Helicoverpa armigera, H. armigera
Subjects: Others > Pest Management
Others > Helicoverpa
Others > Entomology
Depositing User: Mr Ramesh K
Date Deposited: 05 Apr 2018 10:14
Last Modified: 05 Apr 2018 10:15
URI: http://oar.icrisat.org/id/eprint/10532
Official URL: http://dx.doi.org/10.1007/s10863-017-9739-3
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: The present work was carried out at the Insect Rearing Laboratory, Entomology, ICRISAT, Hyderabad. It was supported by Gulbarga University, Gulbarga, India, under financial assistance for Ph.D. students (No. GUG/SC/ST cell/2013-14/375) and Special Assistance Programme (SAP) sanctioned to the Department by Govt. of India.
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