Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation

Siddaiah, C N and Prasanth, K V H and Satyanarayana, N R and Mudili, V and Gupta, V K and Kalagatur, N K and Satyavati, T and Dai, X F and Chen, J Y and Mocan, A and Singh, B P and Srivastava, R K (2018) Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation. Scientific Reports (TSI), 8 (1) (2485). pp. 1-14. ISSN 2045-2322

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Abstract

Downy mildew of pearl millet caused by the biotrophic oomycete Sclerospora graminicola is the most devastating disease which impairs pearl millet production causing huge yield and monetary losses. Chitosan nanoparticles (CNP) were synthesized from low molecular weight chitosan having higher degree of acetylation was evaluated for their efficacy against downy mildew disease of pearl millet caused by Sclerospora graminicola. Laboratory studies showed that CNP seed treatment significantly enhanced pearl millet seed germination percentage and seedling vigor compared to the control. Seed treatment with CNP induced systemic and durable resistance and showed significant downy mildew protection under greenhouse conditions in comparison to the untreated control. Seed treatment with CNP showed changes in gene expression profiles wherein expression of genes of phenylalanine ammonia lyase, peroxidase, polyphenoloxidase, catalase and superoxide dismutase were highly upregulated. CNP treatment resulted in earlier and higher expression of the pathogenesis related proteins PR1 and PR5. Downy mildew protective effect offered by CNP was found to be modulated by nitric oxide and treatment with CNP along with NO inhibitors cPTIO completely abolished the gene expression of defense enzymes and PR proteins. Further, comparative analysis of CNP with Chitosan revealed that the very small dosage of CNP performed at par with recommended dose of Chitosan for downy mildew management.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: UNSPECIFIED
Uncontrolled Keywords: Downy mildew, pearl millet, downy mildew management, seed treatment, seed germination, downy mildew disease, fungi, plant biotechnology, plant science, plant disease management, nitric oxide, nanoparticles
Subjects: Mandate crops > Millets > Pearl Millet
Others > Genetics and Genomics
Others > Plant Growth
Others > Plant Disease
Depositing User: Mr Ramesh K
Date Deposited: 03 Mar 2018 14:56
Last Modified: 11 Apr 2018 07:43
URI: http://oar.icrisat.org/id/eprint/10469
Official URL: http://dx.doi.org/10.1038/s41598-017-19016-z
Projects: UNSPECIFIED
Funders: ICAR
Acknowledgement: The authors are grateful to ICAR (Indian Council of Agricultural Research), New Delhi, India for providing the financial support under AICRP scheme.
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