Proteome analysis of Aspergillus flavus isolate-specific responses to oxidative stress in relationship to aflatoxin production capability

Fountain, J C and Koh, J and Yang, L and Pandey, M K and Nayak, S N and Bajaj, P and Zhuang, W J and Chen, Z Y and Kemerait, R C and Lee, R D and Chen, S and Varshney, R K and Guo, B (2018) Proteome analysis of Aspergillus flavus isolate-specific responses to oxidative stress in relationship to aflatoxin production capability. Scientific Reports (TSI), 8 (1) (3430). pp. 1-14. ISSN 2045-2322

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Abstract

Aspergillus flavus is an opportunistic pathogen of plants such as maize and peanut under conducive conditions such as drought stress resulting in significant aflatoxin production. Drought-associated oxidative stress also exacerbates aflatoxin production by A. flavus. The objectives of this study were to use proteomics to provide insights into the pathogen responses to H2O2-derived oxidative stress, and to identify potential biomarkers and targets for host resistance breeding. Three isolates, AF13, NRRL3357, and K54A with high, moderate, and no aflatoxin production, were cultured in medium supplemented with varying levels of H2O2, and examined using an iTRAQ (Isobaric Tags for Relative and Absolute Quantification) approach. Overall, 1,173 proteins were identified and 220 were differentially expressed (DEPs). Observed DEPs encompassed metabolic pathways including antioxidants, carbohydrates, pathogenicity, and secondary metabolism. Increased lytic enzyme, secondary metabolite, and developmental pathway expression in AF13 was correlated with oxidative stress tolerance, likely assisting in plant infection and microbial competition. Elevated expression of energy and cellular component production in NRRL3357 and K54A implies a focus on oxidative damage remediation. These trends explain isolate-to-isolate variation in oxidative stress tolerance and provide insights into mechanisms relevant to host plant interactions under drought stress allowing for more targeted efforts in host resistance research.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Aspergillus flavus, Aflatoxin production, oxidative stress tolerance, drought stress, Aflatoxins host resistance research, plant pathogen, maize, peanut, oxidative stress, Proteome analysis
Subjects: Others > Abiotic Stress
Others > Drought Tolerance
Others > Plant Genetics
Others > Genetics and Genomics
Others > Plant Pathology
Others > Aflatoxins
Depositing User: Mr Ramesh K
Date Deposited: 03 Mar 2018 08:35
Last Modified: 24 Dec 2018 11:58
URI: http://oar.icrisat.org/id/eprint/10455
Official URL: http://dx.doi.org/10.1038/s41598-018-21653-x
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We thank Ning Zhu, Billy Wilson, and Hui Wang for technical assistance in the laboratory. This work is partially supported by the U.S. Department of Agriculture Agricultural Research Service (USDA-ARS), the Georgia Agricultural Commodity Commission for Corn, the Georgia Peanut Commission, the Peanut Foundation, and AMCOE (Aflatoxin Mitigation Center of Excellence, Chesterfield, MO, USA). This work has also been undertaken as part of the CGIAR Research Program on Grain Legumes and the USAID University Linkages Program between USDA-ARS and ICRISAT. ICRISAT is a member of CGIAR Consortium. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.
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