Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas

Choudhary, N and Bawa, V and Paliwal, R and Singh, B and Bhat, M A and Mir, J I and Gupta, M and Sofi, P A and Thudi, M and Varshney, R K and Mir, R R (2018) Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas. PLoS ONE, 13 (2). pp. 1-12. ISSN 1932-6203

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Abstract

Common bean (Phaseolus vulgaris L.) is one of the most important grain legume crops in the world. The beans grown in north-western Himalayas possess huge diversity for seed color, shape and size but are mostly susceptible to Anthracnose disease caused by seed born fungus Colletotrichum lindemuthianum. Dozens of QTLs/genes have been already identified for this disease in common bean world-wide. However, this is the first report of gene/QTL discovery for Anthracnose using bean germplasm from north-western Himalayas of state Jammu & Kashmir, India. A core set of 96 bean lines comprising 54 indigenous local landraces from 11 hot-spots and 42 exotic lines from 10 different countries were phenotyped at two locations (SKUAST-Jammu and Bhaderwah, Jammu) for Anthracnose resistance. The core set was also genotyped with genome-wide (91) random and trait linked SSR markers. The study of marker-trait associations (MTAs) led to the identification of 10 QTLs/genes for Anthracnose resistance. Among the 10 QTLs/genes identified, two MTAs are stable (BM45 & BM211), two MTAs (PVctt1 & BM211) are major explaining more than 20% phenotypic variation for Anthracnose and one MTA (BM211) is both stable and major. Six (06) genomic regions are reported for the first time, while as four (04) genomic regions validated the already known QTL/gene regions/clusters for Anthracnose. The major, stable and validated markers reported during the present study associated with Anthracnose resistance will prove useful in common bean molecular breeding programs aimed at enhancing Anthracnose resistance of local bean landraces grown in north-western Himalayas of state Jammu and Kashmir.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: UNSPECIFIED
Uncontrolled Keywords: Common bean, Gene identification, QTLs, Himalayan region, Anthracnose disease, Plant disease
Subjects: Others > Plant Disease
Others > Legume Crops
Depositing User: Mr Ramesh K
Date Deposited: 15 Feb 2018 10:05
Last Modified: 15 Feb 2018 10:40
URI: http://oar.icrisat.org/id/eprint/10425
Official URL: http://dx.doi.org/10.1371/journal.pone.0191700
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: The authors are highly thankful to Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India for providing funds under Young Scientist Award (Grant ID No: SB/FT/LS-355/2012) to Dr. Reyazul Rouf Mir. Neeraj Choudhary is thankful to SERB, DST, Govt. of India for providing her fellowship. Thanks are also due to Head Division of Plant Breeding &Genetics and Director Research, SKUASTJammu for facilitating this work.
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