Genome-wide in silico analysis of dehydrins in Sorghum bicolor , Setaria italica and Zea mays and quantitative analysis of dehydrin gene expressions under abiotic stresses in Sorghum bicolor

Nagaraju, M and Reddy, P S and Anil Kumar, S and Kumar, A and Suravajhala, P and Ali, A and Srivastava, R K and Kavi Kishor, P B and Manohar Rao, D (2018) Genome-wide in silico analysis of dehydrins in Sorghum bicolor , Setaria italica and Zea mays and quantitative analysis of dehydrin gene expressions under abiotic stresses in Sorghum bicolor. Plant Gene, 13. pp. 64-75. ISSN 23524073

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Abstract

Dehydrins (DHNs) are highly hydrophilic, thermo stable, calcium dependent chaperons involved in plant developmental processes as well as in diverse abiotic stresses. A systematic survey resulted in the identification of 7 dehydrins (DHNs) in Setaria italica and Zea mays, but 6 in Sorghum bicolor. They are classified into 5 sub-groups, namely YnSKn, SKn, KnS, S, and YnS. DHNs of Sorghum exhibit 1 ortholog with Oryza sativa and Z. mays and 3 with S. italica. Unlike other DHNs, SbDHN5 has been found as an ordered protein with many phosphorylation sites. Network analyses of novel YnS subgroup showed interaction with HSP70 and FKBP genes. In silico promoter analysis revealed the presence of abscisic acid (ABA), drought, salt, low temperature stress-responsive elements. The miRNA target analysis revealed DHNs are targeted by 51 miRNAs responsive to abiotic stresses. High transcript expressions of DHNs were observed in root, stem and leaf compared to inflorescence in S. bicolor. All DHN genes exhibited high levels of expression in stem under cold, heat, salt, and drought stresses. In contrast to other DHNs, the SbDHN2 of YnS subgroup, exhibited the highest expression, under multiple stresses in all the tissues indicating its involvement against a wide array of abiotic stresses.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: UNSPECIFIED
Uncontrolled Keywords: Abiotic stress; DHNs; Phosphorylation sites; qRT-PCR; Sorghum; YnS sub group
Subjects: Others > Abiotic Stress
Mandate crops > Sorghum
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 12 Feb 2018 10:03
Last Modified: 12 Feb 2018 10:22
URI: http://oar.icrisat.org/id/eprint/10419
Official URL: http://dx.doi.org/10.1016/j.plgene.2018.01.004
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: MN is thankful to the UGC, New Delhi, for providing fellowship. PBK is thankful to the CSIR, New Delhi, for providing CSIR-Emeritus Scientist Fellowship. DMR is thankful to the UGC [F.41-828/2012 (SR)], New Delhi, for financial support through UGC-MRP.
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