Kale, S M and Jaganathan, D and Roorkiwal, M and Thudi, M and Purushothaman, R and Praveenkumar, M and Krishnamurthy, L and Kavi Kishor, P B and Gaur, P M and Varshney, R K (2017) Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed weight and drought related traits in chickpea. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.
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Abstract
A “QTL-hotspot” region of 7 Mb size for drought component traits was identified on CaLG04, using a recombinant inbred line (RIL) population (ICC 4958 × ICC 1882) in chickpea. Further, skimbased genotyping by sequencing (GBS) approach with large SNP markers delimited the “QTL-hotspot” region into two sub-regions; viz, “QTL-hotspot_a” of 139.22 kb and “QTL-hotspot_b” of 153.36 kb, on the kabuli draft genome sequence. In order to validate and identify more recombinations in the sub-regions for further refinement, a fine mapping population with 1,911 lines was developed. Flanking markers of the two “QTL-hotspot” sub-regions were converted to KASPar assays and used to screen the fine mapping population consisting of 1,911 lines. As a result, 19 F2:3 recombinant families were identified. These families were phenotyped for seed weight and other drought-related traits. Comparison of genotype and phenotype data identified a genomic region of ~113 Kb size within “QTL-hotspot_a” responsible for 100 seed weight (100SDW) and other drought-related traits in chickpea. Subsequently, a syntenic study between the refined “QTL-hotpsot” region and desi genome identified a 2 Mb region on Ca_LG_4 pseudomolecule. Whole genome re-sequencing (WGRS) analysis of selected lines from each recombinant family identified several non-synonymous and InDel mutations within important candidate genes. Functional validation of these genes will help decipher the mechanism of drought stress tolerance in chickpea.
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Divisions: | Research Program : Genetic Gains |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Fine mapping studies, QTL-hotspot, Drought related traits, Chickpea |
| Subjects: | Others > Abiotic Stress Others > Drought Tolerance Mandate crops > Chickpea Others > Drought Others > Genetics and Genomics |
| Depositing User: | Mr Ramesh K |
| Date Deposited: | 22 Nov 2017 05:40 |
| Last Modified: | 03 Jul 2018 09:05 |
| URI: | http://oar.icrisat.org/id/eprint/10304 |
| Acknowledgement: | UNSPECIFIED |
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