Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

Kaushal, M and Kumar, A and Kaushal, R (2017) Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum. 3 Biotech, 7 (2) (90). pp. 1-9. ISSN 2190-572X

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Abstract

A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35 °C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus. Furthermore, the genome of this isolate was searched for antimicrobial lipopeptide, i.e., ItuD and SrfC genes. The PCR amplification results showed the presence of both these lipopeptide genes in isolate YSPMK11. Iturin A as antifungal compound was identified as major components of fraction through GC/MS. In field experiments, the application of strain YSPMK11 cell suspension (108 CFU/ml) suppressed disease severity by 93% and increased curd yield by 36% which was more that of commercially used fungicide in farmer practices under mid-hills of Himachal Pradesh. Conclusively, our study is first to demonstrate the effect of B. pumilus strain YSPMK11 in suppression of SSR under field conditions and would be employed as an efficient biocontrol agent to replace commercial fungicides in cauliflower cropping system. In addition, the presence of both lipopeptide genes (ItuD and SrfC) and iturin A in this isolate makes him potent strain for biological control application in agriculture.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Bacillus pumilus, Sclerotinia sclerotiorum, 16S rRNA, Disease suppression, Cauliflower production
Subjects:	Others > Agriculture-Farming, Production, Technology, Economics
Depositing User:	Mr Ramesh K
Date Deposited:	16 Nov 2017 10:46
Last Modified:	16 Nov 2017 10:46
URI:	http://oar.icrisat.org/id/eprint/10283
Official URL:	http://dx.doi.org/10.1007/s13205-017-0732-7
Projects:	UNSPECIFIED
Funders:	ICAR
Acknowledgement:	Authors thanks to ICAR, New Delhi for financial support provided to carry out this study.
Links:	Google Scholar

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