Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization

Nayak, S N and Agarwal, G and Pandey, M K and Sudini, H and Jayale, A S and Purohit, S and Desai, A and Wan, L and Guo, B and Liao, B and Varshney, R K (2017) Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization. Scientific Reports, 7 (9659). pp. 1-14. ISSN 2045-2322

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Abstract

Aflatoxin contamination, caused by fungal pathogen Aspergillus flavus, is a major quality and health problem delimiting the trade and consumption of groundnut (Arachis hypogaea L.) worldwide. RNA-seq approach was deployed to understand the host-pathogen interaction by identifying differentially expressed genes (DEGs) for resistance to in-vitro seed colonization (IVSC) at four critical stages after inoculation in J 11 (resistant) and JL 24 (susceptible) genotypes of groundnut. About 1,344.04 million sequencing reads have been generated from sixteen libraries representing four stages in control and infected conditions. About 64% and 67% of quality filtered reads (1,148.09 million) were mapped onto A (A. duranensis) and B (A. ipaёnsis) subgenomes of groundnut respectively. About 101 million unaligned reads each from J 11 and JL 24 were used to map onto A. flavus genome. As a result, 4,445 DEGs including defense-related genes like senescence-associated proteins, resveratrol synthase, 9s-lipoxygenase, pathogenesis-related proteins were identified. In A. flavus, about 578 DEGs coding for growth and development of fungus, aflatoxin biosynthesis, binding, transport, and signaling were identified in compatible interaction. Besides identifying candidate genes for IVSC resistance in groundnut, the study identified the genes involved in host-pathogen cross-talks and markers that can be used in breeding resistant varieties.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Aflatoxin contamination, Host-pathogen, Groundnut, Aflatoxin estimation, Aspergillus infection, Aspergillus flavus infection
Subjects: Others > Pest Management
Mandate crops > Groundnut
Others > Plant Disease
Others > Aflatoxins
Depositing User: Mr Ramesh K
Date Deposited: 05 Sep 2017 04:57
Last Modified: 07 Sep 2017 08:44
URI: http://oar.icrisat.org/id/eprint/10172
Official URL: http://dx.doi.org/10.1038/s41598-017-09260-8
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We acknowledge Dr. Manish Vishwakarma for designing allele specific primers and Ms. Vanika Garg for preparing circos diagram for the manuscript. This research was partially funded by the USAID-US University Collaboration Grant, Peanut & Mycotoxin Innovation Lab (PMIL), National Natural Science Foundation of China (No. 31461143022) funded China-CGIAR Collaborative Research Project and MARS Chocolate North America, USA. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium.
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