Molecular insights into the functional role of nitric oxide (NO) as a signal for plant responses in chickpea

Santisree, P and Bhatnagar-Mathur, P and Sharma, K K (2017) Molecular insights into the functional role of nitric oxide (NO) as a signal for plant responses in chickpea. Functional Plant Biology. pp. 1-17. ISSN 1445-4408

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Abstract

The molecular mechanisms and targets of nitric oxide (NO) are not fully known in plants. Our study reports the first large-scale quantitative proteomic analysis of NO donor responsive proteins in chickpea. Dose response studies carried out using NO donors, sodium nitroprusside (SNP), diethylamine NONOate (DETA) and S-nitrosoglutathione (GSNO) in chickpea genotype ICCV1882, revealed a dose dependent positive impact on seed germination and seedling growth. SNP at 0.1 mM concentration proved to be most appropriate following confirmation using four different chickpea genotypes. while SNP treatment enhanced the percentage of germination, chlorophyll and nitrogen contents in chickpea, addition of NO scavenger, cPTIO reverted its impact under abiotic stresses. Proteome profiling revealed 172 downregulated and 76 upregulated proteins, of which majority were involved in metabolic processes (118) by virtue of their catalytic (145) and binding (106) activity. A few crucial proteins such as S-adenosylmethionine synthase, dehydroascorbate reductase, pyruvate kinase fragment, 1-aminocyclopropane-1-carboxylic acid oxidase, 1-pyrroline-5-carboxylate synthetase were less abundant whereas Bowman-Birk type protease inhibitor, non-specific lipid transfer protein, chalcone synthase, ribulose-1-5-bisphosphate carboxylase oxygenase large subunit, PSII D2 protein were highly abundant in SNP treated samples. This study highlights the protein networks for a better understanding of possible NO induced regulatory mechanisms in plants.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	CGIAR Research Program on Grain Legumes
Uncontrolled Keywords:	Abiotic stress, Gel-free proteomics, Mass spectroscopy, Sodium nitroprusside, Nitric oxide, Chickpea
Subjects:	Others > Molecular Biology Mandate crops > Chickpea Others > Genetics and Genomics
Depositing User:	Mr Ramesh K
Date Deposited:	07 Aug 2017 10:06
Last Modified:	10 Aug 2017 06:41
URI:	http://oar.icrisat.org/id/eprint/10129
Official URL:	http://dx.doi.org/10.1071/FP16324
Projects:	UNSPECIFIED
Funders:	Department of Science and Technology, Government of India & CGIAR Research Program on Grain Legumes
Acknowledgement:	PS thanks ICRISAT for hosting and providing research facilities. This work was undertaken as part of the CGIAR Research Program on Grain Legumes. This work was supported by financial support to PS through the INSPIRE Faculty Award (IFA12-LSPA-08) from the Department of Science and Technology, Government of India and partial funding from the CGIAR Research Program on Grain Legumes to KKS.
Links:	Google Scholar

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