Elicitation of resistance and associated defense responses in Trichoderma hamatum induced protection against pearl millet downy mildew pathogen

Siddaiah, C N and Satyanarayana, N R and Mudili, V and Kumar Gupta, V and Gurunathan, S and Rangappa, S and Huntrike, S S and Srivastava, R K (2017) Elicitation of resistance and associated defense responses in Trichoderma hamatum induced protection against pearl millet downy mildew pathogen. Scientific Reports, 7 (43991). pp. 1-18. ISSN 2045-2322

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Abstract

Endophytic Trichoderma hamatum UoM 13 isolated from pearl millet roots was evaluated for its efficiency to suppress downy mildew disease. Under laboratory conditions, T. hamatum seed treatment significantly enhanced pearl millet seed germination and seedling vigor. T. hamatum seed treatment resulted in systemic and durable immunity against pearl millet downy mildew disease under greenhouse and field conditions. T. hamatum treated seedlings responded to downy mildew infection with high lignification and callose deposition. Analysis of defense enzymes showed that T. hamatum treatment significantly enhanced the activities of glucanase, peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase in comparison to untreated control. RT-PCR analysis revealed differentially expressed transcripts of the defense enzymes and PR-proteins in treated, untreated, and checks, wherein PR-1, PR-5, and cell wall defense HRGPs were significantly over expressed in treated seedlings as against their lower expression in controls. T. hamatum treatment significantly stimulated endogenous salicylic acid (SA) levels and significantly upregulated important SA biosynthesis gene isochorismate synthase. The results indicated that T. hamatum UoM13 treatment induces resistance corresponding to significant over expression of endogenous SA, important defense enzymes, PR-proteins, and HRGPs, suggesting that SA biosynthetic pathway is involved in pearl millet for mounting systemic immunity against downy mildew pathogen.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Pearl millet, Biochemical studies, Gene expression studies, T. hamatum, Downy mildew pathogen, Plant disease
Subjects: Mandate crops > Millets > Pearl Millet
Others > Plant Disease
Depositing User: Mr Ramesh K
Date Deposited: 29 Jun 2017 09:53
Last Modified: 29 Jun 2017 09:54
URI: http://oar.icrisat.org/id/eprint/10065
Official URL: http://dx.doi.org/10.1038/srep43991
Projects: UNSPECIFIED
Funders: ICAR
Acknowledgement: The authors are grateful to ICAR (Indian Council of Agricultural Research), New Delhi, India for providing the financial support under AICRP scheme.
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