Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea

Saxena, R K and Singh, V K and Kale, S M and Tathineni, R and Parupalli, S and Kumar, V and Garg, V and Das, R R and Sharma, M and Yamini, K N and Muniswamy, S and Ghanta, A and Rathore, A and Sameer Kumar, C V and Saxena, K B and Kishor, P B K and Varshney, R K (2017) Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea. Scientific Reports, 7 (1911). pp. 1-11. ISSN 2045-2322

[img]
Preview
PDF (It is an Open Access article) - Published Version
Download (3MB) | Preview

Abstract

Fusarium wilt (FW) is one of the most important biotic stresses causing yield losses in pigeonpea. Genetic improvement of pigeonpea through genomics-assisted breeding (GAB) is an economically feasible option for the development of high yielding FW resistant genotypes. In this context, two recombinant inbred lines (RILs) (ICPB 2049 × ICPL 99050 designated as PRIL_A and ICPL 20096 × ICPL 332 designated as PRIL_B) and one F2 (ICPL 85063 × ICPL 87119) populations were used for the development of high density genetic maps. Genotyping-by-sequencing (GBS) approach was used to identify and genotype SNPs in three mapping populations. As a result, three high density genetic maps with 964, 1101 and 557 SNPs with an average marker distance of 1.16, 0.84 and 2.60 cM were developed in PRIL_A, PRIL_B and F2, respectively. Based on the multi-location and multi-year phenotypic data of FW resistance a total of 14 quantitative trait loci (QTLs) including six major QTLs explaining >10% phenotypic variance explained (PVE) were identified. Comparative analysis across the populations has revealed three important QTLs (qFW11.1, qFW11.2 and qFW11.3) with upto 56.45% PVE for FW resistance. This is the first report of QTL mapping for FW resistance in pigeonpea and identified genomic region could be utilized in GAB.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Genotyping, Genetic maps, QTL mapping, Pigeonpea, Mapping populations, Phenotypic evaluation, QTL analysis, Fusarium wilt
Subjects: Mandate crops > Pigeonpea
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 12 Jun 2017 03:46
Last Modified: 12 Jun 2017 03:46
URI: http://oar.icrisat.org/id/eprint/10043
Official URL: http://dx.doi.org/10.1038/s41598-017-01537-2
Projects: UNSPECIFIED
Funders: United States Agency for International Development (USAID)
Acknowledgement: We thank Abdul Gafoor and Meriga Sudhakar for excellent technical assistance. Authors are thankful to the United States Agency for International Development (USAID), for providing funding support. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium.
Links:

Actions (login required)

View Item View Item