Streptomyces spp., a potential biocontrol agent of charcoal rot of sorghum caused by Macrophomina phaseolina

Alekhya, G and Sharma, R and Gopalakrishnan, S (2016) Streptomyces spp., a potential biocontrol agent of charcoal rot of sorghum caused by Macrophomina phaseolina. Indian Journal of Plant Protection, 44 (2). pp. 222-228. ISSN 0253-4355

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Abstract

Seven strains of Streptomyces spp. (BCA-546, BCA-659, BCA-667, BCA-689, BCA-698, CAI-8 and CAI-133; demonstrated previously to have potential for plant growth-promotion on sorghum and chickpea) were evaluated for their antagonistic potential against Macrophomina phaseolina, causal agent of charcoal rot disease of sorghum, by dual culture assay, secondary metabolite production assay and in greenhouse disease screen. All the seven strains inhibited M. phaseolina in both dual culture as well as secondary metabolite production assays but four of them (BCA-546, BCA-667, BCA-698 and CAI-8) were notable. When these selected four strains were tested for their antifungal activity in greenhouse on sorghum by tooth pick method, BCA-546 and CAI-8 significantly reduced the disease. The stem samples of the control and Streptomyces sp. treated plants were analysed under scanning electron microscope, where the xylem and phloem tissues of the Streptomyces spp. treated plants were found intact compared to that of infected control plants. This study indicates that the selected two Streptomyces strains, BCA-546 and CAI-8, have the potential to control charcoal rot disease in sorghum.

Item Type: Article
Divisions: Research Program : Asia
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Charcoal rot, sorghum, Macrophomina phaseolina, Streptomyces spp., biocontrol
Subjects: Mandate crops > Sorghum
Depositing User: Mr Ramesh K
Date Deposited: 24 May 2017 08:58
Last Modified: 24 May 2017 08:58
URI: http://oar.icrisat.org/id/eprint/10023
Official URL:
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We are thankful to DST-INSPIRE for the financial grant to G Alekhya for her PhD fellowship. This work was undertaken as part of the CGIAR Research Program on Dryland Cereals ICRISAT is a member of CGIAR Consortium. We thank M Lakshman, Associate professor, Ruska Lab, College of Veterinary Science, Rajendranagar, Hyderabad, for SEM analysis and also the staff of the biocontrol unit of ICRISAT, including V Srinivas, A Satya, R Vijayabharathi, PVS Prasad, P Manohar and A Jabbar for their significant inputs in the laboratory and greenhouse studies.
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